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Literature summary for 3.1.1.3 extracted from

  • Fendri, A.; Frikha, F.; Mosbah, H.; Miled, N.; Zouari, N.; Bacha, A.B.; Sayari, A.; Mejdoub, H.; Gargouri, Y.
    Biochemical characterization, cloning, and molecular modelling of chicken pancreatic lipase (2006), Arch. Biochem. Biophys., 451, 149-159.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Colipase required Gallus gallus
additional information CPL presents the interfacial activation phenomenon determined with tripropionin or vinyl ester Gallus gallus
Na-deoxycholate bile salts are required Gallus gallus

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and anaylsis Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
bovine serum albumin amphipathic protein, the inhibition is fully reversible by addition of bile salts and colipase Gallus gallus
Triton X-100 the inhibition is fully reversible by addition of bile salts and colipase Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49401
-
1 * 50000, SDS-PAGE, 1 * 49401, sequence calculation Gallus gallus
50000
-
gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus Q0QJZ3 fragment
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein determination with the anthrone-sulfuric acid method using glucose as standard Gallus gallus

Purification (Commentary)

Purification (Comment) Organism
native enzyme 94.7fold from pancreas by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and again anion exchange chromatography to homogeneity Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9000
-
purified native enzyme, using tributyrin as substrate in the presence of 4 mM Na-deoxycholate and an excess of colipase. Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
olive oil + H2O the enzyme can tolerate the accumulation of long-chain free fatty acids at the interface when olive oil emulsion is used as substrate in the absence of bile salts and colipase Gallus gallus ?
-
?
tributyrin + H2O
-
Gallus gallus dibutyrin + butyrate
-
?
tripropionin + H2O
-
Gallus gallus dipropionin + propionate
-
?
vinyl propionate + H2O
-
Gallus gallus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE, 1 * 49401, sequence calculation Gallus gallus
More building of a 3D structure model of CPL with the catalytic pocket using the human pancreatic lipase as template, overview Gallus gallus

Synonyms

Synonyms Comment Organism
chicken pancreatic lipase
-
Gallus gallus
CPL
-
Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Gallus gallus