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Literature summary for 3.1.1.3 extracted from

  • Kim, K.R.; Kwon, D.Y.; Yoon, S.H.; Kim, W.Y.; Kim, K.H.
    Purification, refolding, and characterization of recombinant Pseudomonas fluorescens lipase (2005), Protein Expr. Purif., 39, 124-129.
    View publication on PubMed
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain BL21 in inclusion bodies Pseudomonas fluorescens

General Stability

General Stability Organism
Ca2+ is important for structural stability of the enzyme Pseudomonas fluorescens

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates the recombinant refolded enzyme, induces secondary structural changes, confers resistance to denaturation by guanidinium hydrochloride and to inactivation by DMSO and trifluoroethanol, Ca2+ is important for structural stability of the enzyme Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas fluorescens the enzyme is responsible for the spoilage of milk ?
-
 ?
additional information Pseudomonas fluorescens SIK W1 the enzyme is responsible for the spoilage of milk ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-
Pseudomonas fluorescens SIK W1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant refolded enzyme 2.5fold from Escherichia coli strain BL21 by gel filtration and ion exchange chromatography Pseudomonas fluorescens

Renatured (Commentary)

Renatured (Comment) Organism
solubilized recombinant enzyme from inclusion bodies after expression in Escherichia coli is refolded by refolding buffer incubation overnight and gel filtration at 4°C, overview Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9264
-
 purified refolded recombinant enzyme Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is responsible for the spoilage of milk Pseudomonas fluorescens ?
-
 ?
additional information the enzyme is responsible for the spoilage of milk Pseudomonas fluorescens SIK W1 ?
-
 ?

Subunits

Subunits Comment Organism
More Ca2+ induces secondary structural changes, Ca2+ is important for structural stability of the enzyme Pseudomonas fluorescens

Synonyms

Synonyms Comment Organism
lipase
-
 Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pseudomonas fluorescens