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Literature summary for 3.1.1.3 extracted from

  • Gilbert, E.J.; Cornish, A.; Jones, C.W.
    Purification and properties of extracellular lipase from Pseudomonas aeruginosa EF2 (1991), J. Gen. Microbiol., 137, 2223-2229.
    View publication on PubMed
Search for more literature for 3.1.1.3

General Stability

General Stability Organism
the purified enzyme is very stable to freezing and thawing Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin weak inhibition Pseudomonas aeruginosa
additional information no inhibition by EDTA at 1 mM Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 the enzyme tends to form aggregates Pseudomonas aeruginosa
29000
-
 gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa EF2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from medium of a Tween 80-limited culture, by anion exchange chromatography and gel filtration Pseudomonas aeruginosa

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also shows 8fold lower esterase activity with 4-nitrophenyl acetate and Tween 80 as substrates Pseudomonas aeruginosa ?
-
 ?
additional information the enzyme also shows 8fold lower esterase activity with 4-nitrophenyl acetate and Tween 80 as substrates Pseudomonas aeruginosa EF2 ?
-
 ?
triolein + H2O olive oil with predominantly triolein, regiospecificity for the 1- and 3-oleoyl residues Pseudomonas aeruginosa diolein + oleate
-
 ?
triolein + H2O olive oil with predominantly triolein, regiospecificity for the 1- and 3-oleoyl residues Pseudomonas aeruginosa EF2 diolein + oleate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
extracellular lipase
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life is 17.5 min Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3000
-
 triolein
-
 Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa
-
-
 4.9