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Literature summary for 3.1.1.3 extracted from
- Tgl4p and Tgl5p, two triacylglycerol lipases of the yeast Saccharomyces cerevisiae are localized to lipid particles (2005), J. Biol. Chem., 280, 37301-37309.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes tgl4 and tgl5, DNA and amino acid sequence determination and analysis, expression of His-tagged isozymes in Pichia pastoris, subcellular localization analysis | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lipid droplet | colocalization of isozymes Tgl4p and Tgl5p | Saccharomyces cerevisiae | 5811 | - |
| additional information | subcellular localization analysis | Saccharomyces cerevisiae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Saccharomyces cerevisiae | the enzyme is required for mobilization of triacylglycerides stored in lipid particles, overview | ? | - |
? | |
| additional information | Saccharomyces cerevisiae BY4741 | the enzyme is required for mobilization of triacylglycerides stored in lipid particles, overview | ? | - |
? | |
| trimyristin + H2O | Saccharomyces cerevisiae | - |
dimyristin + myristate | - |
? | |
| trimyristin + H2O | Saccharomyces cerevisiae BY4741 | - |
dimyristin + myristate | - |
? | |
| tripalmitin + H2O | Saccharomyces cerevisiae | - |
dipalmitin + palmitate | - |
? | |
| tripalmitin + H2O | Saccharomyces cerevisiae BY4741 | - |
dipalmitin + palmitate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
isozymes Tgl4p and Tgl5p | - |
| Saccharomyces cerevisiae BY4741 | - |
isozymes Tgl4p and Tgl5p | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant HIs-tagged isozymes Tgl4p and Tgl5p from Pichia pastoris | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2,3-trihexaicosanoylglycerol + H2O | - |
Saccharomyces cerevisiae | 1,2-dihexaicosanoylglycerol + hexaicosanoate | - |
? | |
| additional information | substrate specificity of isozymes, overview | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | the enzyme is required for mobilization of triacylglycerides stored in lipid particles, overview | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | substrate specificity of isozymes, overview | Saccharomyces cerevisiae BY4741 | ? | - |
? | |
| additional information | the enzyme is required for mobilization of triacylglycerides stored in lipid particles, overview | Saccharomyces cerevisiae BY4741 | ? | - |
? | |
| trilinolein + H2O | - |
Saccharomyces cerevisiae | dilinolein + linoleate | - |
? | |
| trimyristin + H2O | - |
Saccharomyces cerevisiae | dimyristin + myristate | - |
? | |
| trimyristin + H2O | - |
Saccharomyces cerevisiae BY4741 | dimyristin + myristate | - |
? | |
| triolein + H2O | - |
Saccharomyces cerevisiae | diolein + oleate | - |
? | |
| triolein + H2O | - |
Saccharomyces cerevisiae BY4741 | diolein + oleate | - |
? | |
| tripalmitin + H2O | - |
Saccharomyces cerevisiae | dipalmitin + palmitate | - |
? | |
| tripalmitin + H2O | - |
Saccharomyces cerevisiae BY4741 | dipalmitin + palmitate | - |
? | |
| tristearin + H2O | - |
Saccharomyces cerevisiae | distearin + stearate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TAG lipase | - |
Saccharomyces cerevisiae |
| Tgl4p | - |
Saccharomyces cerevisiae |
| Tgl5p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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