KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics study of ROL29 and ROL32 | Rhizopus arrhizus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus arrhizus | P61872 | i.e. Rhizopus delemar | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | cleavage of the N-terminal peptide of ROL29 reduces the activity by 50%, ROL29 occurs after proteolytic cleavage of ROL32 | Rhizopus arrhizus |
Purification (Comment) | Organism |
---|---|
ROL29 to homogeneity | Rhizopus arrhizus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | the catalytic triad is composed of residues Ser173, Asp232, and His285 | Rhizopus arrhizus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dicaprin + H2O | the three dicaprin isomers spread at the air-water interface, ROL29 prefers the distal ester groups of diglyceride isomer 1,3-sn-dicaprin, ROL32 is more stereoselective than ROL29 for the sn-3 position of the 2,3-sn-enantiomer of dicaprin | Rhizopus arrhizus | caprin + caprate | - |
? | |
additional information | substrate specificity, regioselectivity, and stereoselectivity of ROL29 and ROL32, the N-terminal peptide of the enzymes plays a role, overview, interaction of ROL29 and ROL32 with egg-yolk phosphocholine layer and penetration velocity | Rhizopus arrhizus | ? | - |
? | |
tributyrin + H2O | - |
Rhizopus arrhizus | dibutyrin + butyrate | - |
? | |
triolein + H2O | - |
Rhizopus arrhizus | diolein + oleate | - |
? | |
tripropionin + H2O | - |
Rhizopus arrhizus | dipropionin + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure-function relationship and three-dimensional modelling | Rhizopus arrhizus |
Synonyms | Comment | Organism |
---|---|---|
glycerol ester hydrolase | - |
Rhizopus arrhizus |
lipase | - |
Rhizopus arrhizus |
RDL | - |
Rhizopus arrhizus |
ROL29 | - |
Rhizopus arrhizus |
ROL32 | - |
Rhizopus arrhizus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rhizopus arrhizus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Rhizopus arrhizus |