Literature summary for 3.1.1.3 extracted from

Sayari, A.; Frikha, F.; Miled, N.; Mtibaa, H.; Ali, Y.B.; Verger, R.; Gargouri, Y.
N-terminal peptide of Rhizopus oryzae lipase is important for its catalytic properties (2005), FEBS Lett., 579, 976-982.

Search for more literature for 3.1.1.3

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics study of ROL29 and ROL32	Rhizopus arrhizus

Organism

Organism	UniProt	Comment	Textmining
Rhizopus arrhizus	P61872	i.e. Rhizopus delemar	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	cleavage of the N-terminal peptide of ROL29 reduces the activity by 50%, ROL29 occurs after proteolytic cleavage of ROL32	Rhizopus arrhizus

Purification (Commentary)

Purification (Comment)	Organism
ROL29 to homogeneity	Rhizopus arrhizus

Reaction

Reaction	Comment	Organism	Reaction ID
triacylglycerol + H2O = diacylglycerol + a carboxylate	the catalytic triad is composed of residues Ser173, Asp232, and His285	Rhizopus arrhizus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dicaprin + H2O	the three dicaprin isomers spread at the air-water interface, ROL29 prefers the distal ester groups of diglyceride isomer 1,3-sn-dicaprin, ROL32 is more stereoselective than ROL29 for the sn-3 position of the 2,3-sn-enantiomer of dicaprin	Rhizopus arrhizus	caprin + caprate	-	?
additional information	substrate specificity, regioselectivity, and stereoselectivity of ROL29 and ROL32, the N-terminal peptide of the enzymes plays a role, overview, interaction of ROL29 and ROL32 with egg-yolk phosphocholine layer and penetration velocity	Rhizopus arrhizus	?	-	?
tributyrin + H2O	-	Rhizopus arrhizus	dibutyrin + butyrate	-	?
triolein + H2O	-	Rhizopus arrhizus	diolein + oleate	-	?
tripropionin + H2O	-	Rhizopus arrhizus	dipropionin + propionate	-	?

Subunits

Subunits	Comment	Organism
More	structure-function relationship and three-dimensional modelling	Rhizopus arrhizus

Synonyms

Synonyms	Comment	Organism
glycerol ester hydrolase	-	Rhizopus arrhizus
lipase	-	Rhizopus arrhizus
RDL	-	Rhizopus arrhizus
ROL29	-	Rhizopus arrhizus
ROL32	-	Rhizopus arrhizus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rhizopus arrhizus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.2	-	assay at	Rhizopus arrhizus

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Release 2023.1 (January 2023)