Any feedback?
Literature summary for 3.1.1.3 extracted from
- Identification and characterization of a triacylglycerol lipase in Arabidopsis homologous to mammalian acid lipases (2005), FEBS Lett., 579, 6067-6073.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lip1, amino acid sequence comparison, expression of His-tagged enzyme in Escherichia coli, expression in insect cells using the baculovirus system | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of knockout mutant plants which do not show an altered phenotype during germination | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana | the enzyme is involved in but not essential for breakdoen of stored triacylglycerides during germination | ? | - |
? |  |
| triolein + H2O | Arabidopsis thaliana | - |
diolein + oleate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q71DJ5 | precursor; gene lip1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme contains 2 putative N-glycosylation sites | Arabidopsis thaliana |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | tissue expression analysis, no expression in leaf, flower, root, and silique | Arabidopsis thaliana | - |
| seed | germinating, highest activity at the first germination day | Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 45 | - |
recombinant enzyme | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-diolein + H2O | low activity | Arabidopsis thaliana | 1-oleoyl-sn-glycerol + oleate | - |
? | |
| additional information | the enzyme is involved in but not essential for breakdoen of stored triacylglycerides during germination | Arabidopsis thaliana | ? | - |
? | |
| additional information | no activity with olein, digalactosyldiacylglycerol, galactosyldiacylglycerol, phosphatidylcholine, and cholesterol oleate | Arabidopsis thaliana | ? | - |
? | |
| tributyrin + H2O | preferred substrate | Arabidopsis thaliana | dibutyrin + butyrate | - |
? | |
| tricaprylin + H2O | good substrate | Arabidopsis thaliana | dicaprylin + caprylate | - |
? | |
| triolein + H2O | - |
Arabidopsis thaliana | diolein + oleate | - |
? | |
| triolein + H2O | low activity | Arabidopsis thaliana | diolein + oleate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure modelling | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Lip1 | - |
Arabidopsis thaliana |
| TAG lipase | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
recombinant enzyme | Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | recombinant enzyme | Arabidopsis thaliana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
