Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.3 extracted from

  • Finkelstein, A.E.; Strawich, E.S.; Sonnino, S.
    Characterization and partial purification of a lipase from Pseudomonas aeruginosa (1970), Biochim. Biophys. Acta, 206, 380-391.
    View publication on PubMed
Search for more literature for 3.1.1.3

General Stability

General Stability Organism
lyophilization of the crude enzyme leads to only slight loss of activity Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 50% inhibition at 2.5 mM Pseudomonas aeruginosa
EDTA 60% inhibition at 1 mM, completely reversible by 5 mM MgSO4 Pseudomonas aeruginosa
eserine 27% inhibition at 5 mM Pseudomonas aeruginosa
additional information no inhibition of extracellular enzyme by 1 M NaCl, 0.1 mg/ml heparin, and SH-reagents 4-hydroymercuribenzoate, 2-mercaptoethanol, iodoacetate, sodium diphosphate, and NaF Pseudomonas aeruginosa
protamine 50% inhibition at 2 mg/ml Pseudomonas aeruginosa
sodium taurocholate 67% inhibition at 5 mM Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the extracellular enzyme form is secreted to the culture medium Pseudomonas aeruginosa
-
-
intracellular second, minor enzyme form Pseudomonas aeruginosa 5622
-

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 strain 10145, ATCC
-
Pseudomonas aeruginosa ATCC 10145
-
 strain 10145, ATCC
-

Purification (Commentary)

Purification (Comment) Organism
extracellular enzyme 35fold from culture medium by gel filtration and disc electrophoresis Pseudomonas aeruginosa

Source Tissue

Source Tissue Comment Organism Textmining
additional information extracellular enzyme expression during growth phases, profile Pseudomonas aeruginosa
-

Storage Stability

Storage Stability Organism
-20°C, crude enzyme, stable for at least one month with repeated freezing and thawing Pseudomonas aeruginosa
22°C, room temperature, the crude enzyme is stable for a few hours Pseudomonas aeruginosa
4°C, overnight, loss of 60% activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acylglycerol + H2O
-
 Pseudomonas aeruginosa glycerol + a carboxylate
-
 ?
acylglycerol + H2O
-
 Pseudomonas aeruginosa ATCC 10145 glycerol + a carboxylate
-
 ?
diacylglycerol + H2O
-
 Pseudomonas aeruginosa acylglycerol + a carboxylate
-
 ?
diacylglycerol + H2O
-
 Pseudomonas aeruginosa ATCC 10145 acylglycerol + a carboxylate
-
 ?
additional information the enzyme is unspecific, but prefers substrates with shorter side chains, exo-enzyme Pseudomonas aeruginosa ?
-
 ?
additional information the enzyme is unspecific, but prefers substrates with shorter side chains, exo-enzyme Pseudomonas aeruginosa ATCC 10145 ?
-
 ?
triacylglycerol + H2O
-
 Pseudomonas aeruginosa diacylglycerol + a carboxylate
-
 ?
triacylglycerol + H2O
-
 Pseudomonas aeruginosa ATCC 10145 diacylglycerol + a carboxylate
-
 ?
tributyrin + H2O
-
 Pseudomonas aeruginosa dibutyrin + butyrate
-
 ?
tributyrin + H2O
-
 Pseudomonas aeruginosa ATCC 10145 dibutyrin + butyrate
-
 ?

Synonyms

Synonyms Comment Organism
glycerol ester hydrolase
-
 Pseudomonas aeruginosa
lipase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 extracellular enzyme Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 50 min, 50% remaining activity Pseudomonas aeruginosa
60
-
 3 min, 50% remaining activity Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.9
-
 extracellular enzyme Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
5 11
-
 Pseudomonas aeruginosa