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Literature summary for 3.1.1.3 extracted from

  • Sharma, R.; Soni, S.K.; Vohra, R.M.; Gupta, L.K.; Gupta, J.K.
    Purification and characterization of a thermostable alkaline lipase from a new thermophilic Bacillus sp. RSJ-1 (2002), Process Biochem., 37, 1075-1084.
No PubMed abstract available
Search for more literature for 3.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
additional information not affected by EDTA Bacillus sp. (in: Bacteria)

Application

Application Comment Organism
detergent the enzyme is usable with laundry detergents Bacillus sp. (in: Bacteria)

General Stability

General Stability Organism
Ca2+ stabilizes the enzyme at concentration of 1-10 mM with decreasing effect at higher concentration, 100% activity at 1 mM, 4% activity at 10 mM Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Bacillus sp. (in: Bacteria)
ammonium persulfate
-
 Bacillus sp. (in: Bacteria)
ascorbic acid
-
 Bacillus sp. (in: Bacteria)
Co2+
-
 Bacillus sp. (in: Bacteria)
Cs+
-
 Bacillus sp. (in: Bacteria)
Cu2+
-
 Bacillus sp. (in: Bacteria)
deoxycholate
-
 Bacillus sp. (in: Bacteria)
K+
-
 Bacillus sp. (in: Bacteria)
Mn2+
-
 Bacillus sp. (in: Bacteria)
additional information not affected by EDTA Bacillus sp. (in: Bacteria)
Ni2+ slight inhibition Bacillus sp. (in: Bacteria)
potassium iodide
-
 Bacillus sp. (in: Bacteria)
Sn2+
-
 Bacillus sp. (in: Bacteria)
Tween-20
-
 Bacillus sp. (in: Bacteria)
Zn2+
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 4-nitrophenyl butyrate pH 8.0, 50°C Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Bacillus sp. (in: Bacteria)
additional information the enzyme is no metalloenzyme, not affected by citrate, Ba2+, K+, and Mg2+ Bacillus sp. (in: Bacteria)
Na+ slight activation Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 thermostable alkaline lipase
-
Bacillus sp. (in: Bacteria) RSJ-1
-
 thermostable alkaline lipase
-

Oxidation Stability

Oxidation Stability Organism
enzyme retains 93% activity at 0.1% H2O2, 74% activity at 0.2% H2O2, and 47% activity at 1% H2O2 Bacillus sp. (in: Bacteria)

Purification (Commentary)

Purification (Comment) Organism
to homogeneity from culture supernatant, 201fold Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
428.1
-
 purified enzyme, substrate 4-nitrophenyl butyrate Bacillus sp. (in: Bacteria)

Storage Stability

Storage Stability Organism
enzyme is stable in nonionic detergents such as Triton X-100, Tween-80, and Tween-20, but is less stable in presence of anionic detergents e.g. deoxycholate Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Bacillus sp. (in: Bacteria) RSJ-1 4-nitrophenol + butyrate
-
 ?
triolein + H2O
-
 Bacillus sp. (in: Bacteria) diolein + oleate
-
 ?
triolein + H2O
-
 Bacillus sp. (in: Bacteria) RSJ-1 diolein + oleate
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
lipase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable for 60 min, after 120 min 90% remaining activity Bacillus sp. (in: Bacteria)
55
-
 half-life 240 min Bacillus sp. (in: Bacteria)
60
-
 half-life 150 min Bacillus sp. (in: Bacteria)
65
-
 half-life 90 min Bacillus sp. (in: Bacteria)
70
-
 half-life 45 min Bacillus sp. (in: Bacteria)
75
-
 half-life 30 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
5 12 12% of maximal activity at pH 5.0, 18% of maximal activity at pH 12.0 Bacillus sp. (in: Bacteria)