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Literature summary for 3.1.1.3 extracted from
- Purification and properties of thermostable lipase from a thermophilic Bacillus stearothermophilus MC7 (2003), J. Mol. Catal. B, 22, 307-313.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sorbitol | 50%, 30 min, 70°C, pH 8.5, 10% activation | Geobacillus stearothermophilus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | possible industrial application of the enzyme in continuous processes due to effective immobilization on diverse materials, overview | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5 mM, 30 min, 70°C, pH 8.5, 65% inhibition | Geobacillus stearothermophilus | |
| Cu2+ | 5 mM, 30 min, 70°C, pH 8.5, complete inhibition | Geobacillus stearothermophilus | |
| dithiothreitol | 5 mM, 30 min, 70°C, pH 8.5, 25% inhibition | Geobacillus stearothermophilus | |
| Fe2+ | 5 mM, 30 min, 70°C, pH 8.5, 90% inhibition | Geobacillus stearothermophilus | |
| Hg2+ | 5 mM, 30 min, 70°C, pH 8.5, 55% inhibition | Geobacillus stearothermophilus | |
| Li+ | 5 mM, 30 min, 70°C, pH 8.5, 15% inhibition | Geobacillus stearothermophilus | |
| Mg2+ | 5 mM, 30 min, 70°C, pH 8.5, 25% inhibition | Geobacillus stearothermophilus | |
| Mn2+ | 5 mM, 30 min, 70°C, pH 8.5, 25% inhibition | Geobacillus stearothermophilus | |
| additional information | no inhibition by iodoacetamide, K+, Na+ at 5 mM | Geobacillus stearothermophilus | |
| N-bromosuccinimide | 5 mM, 30 min, 70°C, pH 8.5, 55% inhibition | Geobacillus stearothermophilus | |
| p-chloromercuribenzoate | 5 mM, 30 min, 70°C, pH 8.5, 70% inhibition | Geobacillus stearothermophilus | |
| PMSF | 5 mM, 30 min, 70°C, pH 8.5, complete inhibition | Geobacillus stearothermophilus | |
| Zn2+ | 5 mM, 30 min, 70°C, pH 8.5, 85% inhibition | Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
4-nitrophenyl palmitate | pH 8.5, 60°C | Geobacillus stearothermophilus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Geobacillus stearothermophilus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10% stimulation at 5 mM | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62500 | - |
x * 62500, SDS-PAGE | Geobacillus stearothermophilus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | 50%, 30 min, 70°C, pH 8.5, loss of 60% activity | Geobacillus stearothermophilus |
| Butanol | 50%, 30 min, 70°C, pH 8.5, complete loss of activity | Geobacillus stearothermophilus |
| chloroform | 50%, 30 min, 70°C, pH 8.5, complete loss of activity | Geobacillus stearothermophilus |
| diethyl ether | 50%, 30 min, 70°C, pH 8.5, complete loss of activity | Geobacillus stearothermophilus |
| Ethanol | 50%, 30 min, 70°C, pH 8.5, loss of 70% activity | Geobacillus stearothermophilus |
| Glycerol | 50%, 30 min, 70°C, pH 8.5, loss of 60% activity | Geobacillus stearothermophilus |
| isopropanol | 50%, 30 min, 70°C, pH 8.5, loss of 60% activity | Geobacillus stearothermophilus |
| Methanol | 50%, 30 min, 70°C, pH 8.5, loss of 20% activity | Geobacillus stearothermophilus |
| n-hexane | 50%, 30 min, 70°C, pH 8.5, loss of 85% activity | Geobacillus stearothermophilus |
| sorbitol | 50%, 30 min, 70°C, pH 8.5, 10% activation | Geobacillus stearothermophilus |
| toluol | 50%, 30 min, 70°C, pH 8.5, complete loss of activity | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
thermostable lipase | - |
| Geobacillus stearothermophilus MC 7 | - |
thermostable lipase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 19.25fold, from culture supernatant | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.9 | - |
purified enzyme, substrate 4-nitrophenyl palmitate | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Geobacillus stearothermophilus MC 7 | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl palmitate + H2O | best substrate | Geobacillus stearothermophilus | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | best substrate | Geobacillus stearothermophilus MC 7 | 4-nitrophenol + palmitate | - |
? | |
| DL-alpha-palmitin + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
| DL-alpha-stearin + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
| additional information | the enzyme also possesses esterase activity | Geobacillus stearothermophilus | ? | - |
? | |
| additional information | the enzyme also possesses esterase activity | Geobacillus stearothermophilus MC 7 | ? | - |
? | |
| olive oil + H2O | emulsion | Geobacillus stearothermophilus | ? | - |
? | |
| triacetin + H2O | - |
Geobacillus stearothermophilus | diacetin + acetate | - |
? | |
| tributyrin + H2O | best substrate | Geobacillus stearothermophilus | dibutyrin + butyrate | - |
? | |
| tributyrin + H2O | best substrate | Geobacillus stearothermophilus MC 7 | dibutyrin + butyrate | - |
? | |
| tricaprin + H2O | - |
Geobacillus stearothermophilus | dicaprin + capriate | - |
? | |
| trilaurin + H2O | - |
Geobacillus stearothermophilus | dilaurin + laurate | - |
? | |
| triolein + H2O | - |
Geobacillus stearothermophilus | diolein + oleate | - |
? | |
| triolein + H2O | - |
Geobacillus stearothermophilus MC 7 | diolein + oleate | - |
? | |
| tripalmitin + H2O | - |
Geobacillus stearothermophilus | dipalmitin + palmitate | - |
? | |
| tristearin + H2O | - |
Geobacillus stearothermophilus | distearin + stearate | - |
? | |
| Tween 80 + H2O | i.e. poly(oxyethylene) sorbitan monooleate | Geobacillus stearothermophilus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62500, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 80 | - |
Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 90 | - |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
purified enzyme: half-life is 30 min, crude enzyme: half-life is 3 h | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | - |
Geobacillus stearothermophilus |
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