Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Acinetobacter sp. | |
Co2+ | - |
Acinetobacter sp. | |
Fe3+ | - |
Acinetobacter sp. | |
Mg2+ | slight activation | Acinetobacter sp. | |
Mn2+ | slight activation | Acinetobacter sp. | |
Rb+ | - |
Acinetobacter sp. |
General Stability | Organism |
---|---|
Ca2+ stabilizes the enzyme, no loss of activity after 30 h at 30°C in presence of Ca2+, 70% loss after 30 h at 30°C of activity in absence of Ca2+ | Acinetobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition | Acinetobacter sp. | |
EDTA | strong, about 90% inhibition at 1 mM | Acinetobacter sp. | |
Hg2+ | strong inhibition | Acinetobacter sp. | |
additional information | no inhibition by 2-mercaptoethanol, dithiothreitol, and Zn2+ | Acinetobacter sp. | |
PMSF | slight inhibition | Acinetobacter sp. | |
Pyridine | rapid deactivation | Acinetobacter sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | LipA, secreted to the medium | Acinetobacter sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Acinetobacter sp. |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | loss of about 25% activity at 30% v/v after 1 h at 30°C | Acinetobacter sp. |
dimethylformamide | loss of about 10% activity at 30% v/v after 1 h at 30°C | Acinetobacter sp. |
additional information | the enzyme is only very slightly affected by isopropanol and acetone | Acinetobacter sp. |
Pyridine | rapid complete deactivation | Acinetobacter sp. |
tetrahydrofuran | loss of about 90% activity at 30% v/v after 1 h at 30°C | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
LipA | - |
Acinetobacter sp. RAG-1 | - |
LipA | - |
Purification (Comment) | Organism |
---|---|
from culture medium, 10.7fold | Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
412 | - |
purified enzyme | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | low activity | Acinetobacter sp. | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | low activity | Acinetobacter sp. RAG-1 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Acinetobacter sp. RAG-1 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | best substrate | Acinetobacter sp. | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | best substrate | Acinetobacter sp. RAG-1 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl octanoate + H2O | best substrate | Acinetobacter sp. | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl octanoate + H2O | best substrate | Acinetobacter sp. RAG-1 | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl stearate + H2O | - |
Acinetobacter sp. | 4-nitrophenol + stearate | - |
? | |
additional information | substrate specificity, enzyme prefers substrates of medium- and long-chain fatty acids | Acinetobacter sp. | ? | - |
? | |
additional information | substrate specificity, enzyme prefers substrates of medium- and long-chain fatty acids | Acinetobacter sp. RAG-1 | ? | - |
? | |
tributyrin + H2O | - |
Acinetobacter sp. | dibutyrin + butyrate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Acinetobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
LipA | - |
Acinetobacter sp. |
lipase | - |
Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Acinetobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 33% of maximal activity at 30°C, about 50% of maximal activity at 70°C | Acinetobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
active up to | Acinetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Acinetobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | high activity | Acinetobacter sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Acinetobacter sp. | isoelectric focusing | - |
5.9 |