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Literature summary for 3.1.1.3 extracted from

  • Neugnot, V.; Moulin, G.; Dubreucq, E.; Bigey, F.
    The lipase/acyltransferase from Candida parapsilosis. Molecular cloning and characterization of purified recombinant enzymes (2002), Eur. J. Biochem., 269, 1734-1745.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
2 orfs: CpLIP1 and CpLIP2, DNA and amino acid sequences determination and analysis, expression in Saccharomyces cerevisiae of both forms as C-terminally His-tagged proteins, only CpLIP2 encodes for an active enzyme Candida parapsilosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48900
-
x * 48900, Lip1, sequence calculation, x * 49100-54000, Lip2, sequence calculation and SDS-PAGE Candida parapsilosis

Organism

Organism UniProt Comment Textmining
Candida parapsilosis Q8NIN8 2 orfs: CpLIP1 and CpLIP2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein Lip2: 2 potential N-glycosylation sites at positions 180 and 231, Lip1: 5 potential N-glycosylation sites at positions 92, 180, 231, 261, and 410 Candida parapsilosis

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged Lip2 from Saccharomyces cerevisiae, 85fold Candida parapsilosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80
-
purified recombinant Lip2 Candida parapsilosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme preferentially performs transfer reactions such as alcoholysis in presence of suitable nucleophiles other than water, and catalyzes the hydrolysis of water insoluble medium-chain triacylglyceride substrates Candida parapsilosis ?
-
?

Subunits

Subunits Comment Organism
? x * 48900, Lip1, sequence calculation, x * 49100-54000, Lip2, sequence calculation and SDS-PAGE Candida parapsilosis

Synonyms

Synonyms Comment Organism
acyltransferase
-
Candida parapsilosis
LIP
-
Candida parapsilosis
lipase
-
Candida parapsilosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
plate assay at Candida parapsilosis
50
-
in vitro assay at Candida parapsilosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
plate and in vitro assay at Candida parapsilosis

pI Value

Organism Comment pI Value Maximum pI Value
Candida parapsilosis Lip1, calculated
-
4.2
Candida parapsilosis Lip2, calculated
-
5.5