Cloned (Comment) | Organism |
---|---|
2 orfs: CpLIP1 and CpLIP2, DNA and amino acid sequences determination and analysis, expression in Saccharomyces cerevisiae of both forms as C-terminally His-tagged proteins, only CpLIP2 encodes for an active enzyme | Candida parapsilosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48900 | - |
x * 48900, Lip1, sequence calculation, x * 49100-54000, Lip2, sequence calculation and SDS-PAGE | Candida parapsilosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida parapsilosis | Q8NIN8 | 2 orfs: CpLIP1 and CpLIP2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | Lip2: 2 potential N-glycosylation sites at positions 180 and 231, Lip1: 5 potential N-glycosylation sites at positions 92, 180, 231, 261, and 410 | Candida parapsilosis |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged Lip2 from Saccharomyces cerevisiae, 85fold | Candida parapsilosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
80 | - |
purified recombinant Lip2 | Candida parapsilosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme preferentially performs transfer reactions such as alcoholysis in presence of suitable nucleophiles other than water, and catalyzes the hydrolysis of water insoluble medium-chain triacylglyceride substrates | Candida parapsilosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48900, Lip1, sequence calculation, x * 49100-54000, Lip2, sequence calculation and SDS-PAGE | Candida parapsilosis |
Synonyms | Comment | Organism |
---|---|---|
acyltransferase | - |
Candida parapsilosis |
LIP | - |
Candida parapsilosis |
lipase | - |
Candida parapsilosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
plate assay at | Candida parapsilosis |
50 | - |
in vitro assay at | Candida parapsilosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
plate and in vitro assay at | Candida parapsilosis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Candida parapsilosis | Lip1, calculated | - |
4.2 |
Candida parapsilosis | Lip2, calculated | - |
5.5 |