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Literature summary for 3.1.1.3 extracted from

  • Jinwal, U.K.; Roy, U.; Chowdhury, A.R.; Bhaduri, A.P.; Roy, P.K.
    Purification and characterization of an alkaline lipase from a newly isolated Pseudomonas mendocina PK-12CS and chemoselective hydrolysis of fatty acid ester (2003), Bioorg. Med. Chem., 11, 1041-1046.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme performs chemically selective hydrolysis, synthesis of optically active substances from achiral or racemic compounds Pseudomonas mendocina

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 28% inhibition at 1 mM Pseudomonas mendocina
Cu2+ 52% inhibition at 1 mM Pseudomonas mendocina
diethyldicarbonate 65% inhibition at 0.1 mM, complete inhibition at 1 mM Pseudomonas mendocina
EDTA 38% inhibition at 1 mM Pseudomonas mendocina
Fe3+ 97% inhibition at 1 mM Pseudomonas mendocina
Hg2+ complete inhibition at 1 mM Pseudomonas mendocina
Mn2+ 17% inhibition at 1 mM Pseudomonas mendocina
additional information no inhibition by PMSF, no inhibition by Tween 20, Tween 80, and Triton X-100, negligible inhibition by Zn2+ Pseudomonas mendocina
SDS 95% inhibition at 0.4% Pseudomonas mendocina

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion to the culture medium Pseudomonas mendocina
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 20% activation at 1 mM Pseudomonas mendocina
additional information negligible stimulation by Na+ and Ca2+ at 1 mM Pseudomonas mendocina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, SDS-PAGE Pseudomonas mendocina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas mendocina Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource ?
-
?
additional information Pseudomonas mendocina PK-12CS Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource ?
-
?
tributyrin + H2O Pseudomonas mendocina
-
dibutyrin + butyrate
-
?
tributyrin + H2O Pseudomonas mendocina PK-12CS
-
dibutyrin + butyrate
-
?
triolein + H2O Pseudomonas mendocina
-
diolein + oleate
-
?
triolein + H2O Pseudomonas mendocina PK-12CS
-
diolein + oleate
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the enzyme shows good stability in water-miscible and immiscible solvents, overview Pseudomonas mendocina

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina
-
alkaline lipase
-
Pseudomonas mendocina PK-12CS
-
alkaline lipase
-

Purification (Commentary)

Purification (Comment) Organism
from culture supernatant to homogeneity, 241fold Pseudomonas mendocina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.28
-
purified enzyme Pseudomonas mendocina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme shows a preference for long-chain fatty acid triacylglyceride substrates and natural oils like olive oil, soybean oil, mustard oil, coconut oil, and best almond oil, substrate specificty overview Pseudomonas mendocina ?
-
?
additional information Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource Pseudomonas mendocina ?
-
?
additional information enzyme shows a preference for long-chain fatty acid triacylglyceride substrates and natural oils like olive oil, soybean oil, mustard oil, coconut oil, and best almond oil, substrate specificty overview Pseudomonas mendocina PK-12CS ?
-
?
additional information Pseudomonas mendocina PK-12CS is able to grow on olive oil, soybean oil, tributyrin and triolein as sole carbon and energysource Pseudomonas mendocina PK-12CS ?
-
?
tributyrin + H2O
-
Pseudomonas mendocina dibutyrin + butyrate
-
?
tributyrin + H2O
-
Pseudomonas mendocina PK-12CS dibutyrin + butyrate
-
?
trilinolin + H2O
-
Pseudomonas mendocina dilinolin + linolate
-
?
triolein + H2O
-
Pseudomonas mendocina diolein + oleate
-
?
triolein + H2O
-
Pseudomonas mendocina PK-12CS diolein + oleate
-
?
tripalmitin + H2O
-
Pseudomonas mendocina dipalmitin + palmitate
-
?
tripalmitin + H2O
-
Pseudomonas mendocina PK-12CS dipalmitin + palmitate
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Pseudomonas mendocina

Synonyms

Synonyms Comment Organism
alkaline lipase
-
Pseudomonas mendocina
PK-12CS lipase
-
Pseudomonas mendocina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Pseudomonas mendocina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
room temperature, stable for more than one month Pseudomonas mendocina
45
-
1 h, loss of 50% activity Pseudomonas mendocina
57
-
1 h, loss of 75% activity Pseudomonas mendocina
60
-
half-life is 10 min Pseudomonas mendocina
65
-
half-life is 4 min Pseudomonas mendocina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas mendocina

pH Range

pH Minimum pH Maximum Comment Organism
7.6 8.6 37°C, activity drops sharply below pH 7.6 and above pH 8.6 Pseudomonas mendocina