Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of the N-terminally His-tagged mature enzyme in Escherichia coli DH5alpha | Staphylococcus warneri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 3% remaining activity at 5 mM | Staphylococcus warneri |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Staphylococcus warneri | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Staphylococcus warneri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45500 | - |
x * 45500, SDS-PAGE | Staphylococcus warneri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus warneri | - |
SWL2, lipase 2 | - |
Staphylococcus warneri 863 | - |
SWL2, lipase 2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | enzyme is organized as a prepro-protein | Staphylococcus warneri |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Staphylococcus warneri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.4 | - |
purified recombinant enzyme, substrate 2-hexandecanoylthio-ethane-1-phosphocholine | Staphylococcus warneri |
25 | - |
purified recombinant enzyme, substrate 4-nitrophenyl butyrate | Staphylococcus warneri |
115 | - |
purified recombinant enzyme, substrate 1,2-dioctanoyl-sn-glycero-3-phosphocholine | Staphylococcus warneri |
123 | - |
purified recombinant enzyme, substrate 1,2-dioctanoyl-sn-glycero-3-phosphoglycol | Staphylococcus warneri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O | - |
Staphylococcus warneri | ? | - |
? | |
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O | - |
Staphylococcus warneri 863 | ? | - |
? | |
1,2-dioctanoyl-sn-glycero-3-phosphoglycol + H2O | - |
Staphylococcus warneri | ? | - |
? | |
2-hexandecanoylthio-ethane-1-phosphocholine + H2O | - |
Staphylococcus warneri | ? | - |
? | |
2-hexandecanoylthio-ethane-1-phosphocholine + H2O | - |
Staphylococcus warneri 863 | ? | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Staphylococcus warneri | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Staphylococcus warneri 863 | 4-nitrophenol + butyrate | - |
? | |
additional information | substrate specificity, the enzyme strongly prefers short-chain substrates, the enzyme SWL2 also shows phospholipase activity | Staphylococcus warneri | ? | - |
? | |
additional information | substrate specificity, the enzyme strongly prefers short-chain substrates, the enzyme SWL2 also shows phospholipase activity | Staphylococcus warneri 863 | ? | - |
? | |
triolein + H2O | low activity | Staphylococcus warneri | diolein + oleate | - |
? | |
triolein + H2O | low activity | Staphylococcus warneri 863 | diolein + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45500, SDS-PAGE | Staphylococcus warneri |
Synonyms | Comment | Organism |
---|---|---|
lipase 2 | - |
Staphylococcus warneri |
SWL2 | - |
Staphylococcus warneri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
about, broad optimum | Staphylococcus warneri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Staphylococcus warneri |