Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.3 extracted from

  • van Kampen, M.D.; Rosenstein, R.; Gotz, F.; Egmond, M.R.
    Cloning, purification and characterisation of Staphylococcus warneri lipase 2 (2001), Biochim. Biophys. Acta, 1544, 229-241.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of the N-terminally His-tagged mature enzyme in Escherichia coli DH5alpha Staphylococcus warneri

Inhibitors

Inhibitors Comment Organism Structure
EDTA 3% remaining activity at 5 mM Staphylococcus warneri

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Staphylococcus warneri
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Staphylococcus warneri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
x * 45500, SDS-PAGE Staphylococcus warneri

Organism

Organism UniProt Comment Textmining
Staphylococcus warneri
-
SWL2, lipase 2
-
Staphylococcus warneri 863
-
SWL2, lipase 2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information enzyme is organized as a prepro-protein Staphylococcus warneri

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Staphylococcus warneri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.4
-
purified recombinant enzyme, substrate 2-hexandecanoylthio-ethane-1-phosphocholine Staphylococcus warneri
25
-
purified recombinant enzyme, substrate 4-nitrophenyl butyrate Staphylococcus warneri
115
-
purified recombinant enzyme, substrate 1,2-dioctanoyl-sn-glycero-3-phosphocholine Staphylococcus warneri
123
-
purified recombinant enzyme, substrate 1,2-dioctanoyl-sn-glycero-3-phosphoglycol Staphylococcus warneri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
Staphylococcus warneri ?
-
?
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
Staphylococcus warneri 863 ?
-
?
1,2-dioctanoyl-sn-glycero-3-phosphoglycol + H2O
-
Staphylococcus warneri ?
-
?
2-hexandecanoylthio-ethane-1-phosphocholine + H2O
-
Staphylococcus warneri ?
-
?
2-hexandecanoylthio-ethane-1-phosphocholine + H2O
-
Staphylococcus warneri 863 ?
-
?
4-nitrophenyl butyrate + H2O
-
Staphylococcus warneri 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Staphylococcus warneri 863 4-nitrophenol + butyrate
-
?
additional information substrate specificity, the enzyme strongly prefers short-chain substrates, the enzyme SWL2 also shows phospholipase activity Staphylococcus warneri ?
-
?
additional information substrate specificity, the enzyme strongly prefers short-chain substrates, the enzyme SWL2 also shows phospholipase activity Staphylococcus warneri 863 ?
-
?
triolein + H2O low activity Staphylococcus warneri diolein + oleate
-
?
triolein + H2O low activity Staphylococcus warneri 863 diolein + oleate
-
?

Subunits

Subunits Comment Organism
? x * 45500, SDS-PAGE Staphylococcus warneri

Synonyms

Synonyms Comment Organism
lipase 2
-
Staphylococcus warneri
SWL2
-
Staphylococcus warneri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
about, broad optimum Staphylococcus warneri

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
Staphylococcus warneri