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Literature summary for 3.1.1.3 extracted from
- Substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydolyze poly(omega-hydroxyalkanoates) (1995), Appl. Environ. Microbiol., 61, 3113-3118.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
| extracellular | - |
Pseudomonas fluorescens | - |
- |
| extracellular | - |
Pseudomonas aeruginosa | - |
- |
| extracellular | - |
Pseudomonas alcaligenes | - |
- |
| extracellular | - |
Burkholderia cepacia | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19400 | - |
x * 19400, SDS-PAGE | Bacillus subtilis |
| 29000 | - |
x * 29000, SDS-PAGE | Pseudomonas aeruginosa |
| 30000 | - |
x * 30000, SDS-PAGE | Pseudomonas alcaligenes |
| 33000 | - |
x * 33000, SDS-PAGE | Burkholderia cepacia |
| 48000 | - |
x * 48000, recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Burkholderia cepacia | - |
- |
- |
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas alcaligenes | - |
- |
- |
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
- |
Pseudomonas aeruginosa |
- |
Pseudomonas alcaligenes |
- |
Burkholderia cepacia |
| recombinant enzyme | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O | - |
Bacillus subtilis | ? | - |
? |  |
| 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
| 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O | - |
Pseudomonas alcaligenes | ? | - |
? | |
| 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O | - |
Burkholderia cepacia | ? | - |
? | |
| 1,2-dilauryl-rac-glycero-3-glutaric acid resorufinester + H2O | - |
Pseudomonas fluorescens GK13 | ? | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Bacillus subtilis | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Pseudomonas alcaligenes | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Burkholderia cepacia | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Pseudomonas fluorescens GK13 | 4-nitrophenol + palmitate | - |
? | |
| additional information | substrate specificity of the extracellular enzyme | Pseudomonas alcaligenes | ? | - |
? | |
| additional information | substrate specificity of the extracellular enzyme | Burkholderia cepacia | ? | - |
? | |
| additional information | substrate specificity of the extracellular enzyme, the enzyme shows high activity | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | substrate specificity of the extracellular enzyme, the enzyme shows low activity | Bacillus subtilis | ? | - |
? | |
| additional information | substrate specificity of the recombinant extracellular enzyme overexpressed in Escherichia coli | Pseudomonas fluorescens | ? | - |
? | |
| additional information | substrate specificity of the recombinant extracellular enzyme overexpressed in Escherichia coli | Pseudomonas fluorescens GK13 | ? | - |
? | |
| triolein + H2O | - |
Bacillus subtilis | diolein + oleate | - |
? | |
| triolein + H2O | - |
Pseudomonas fluorescens | diolein + oleate | - |
? | |
| triolein + H2O | - |
Pseudomonas aeruginosa | diolein + oleate | - |
? | |
| triolein + H2O | - |
Pseudomonas alcaligenes | diolein + oleate | - |
? | |
| triolein + H2O | - |
Burkholderia cepacia | diolein + oleate | - |
? | |
| triolein + H2O | - |
Pseudomonas fluorescens GK13 | diolein + oleate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Pseudomonas alcaligenes |
| ? | x * 29000, SDS-PAGE | Pseudomonas aeruginosa |
| ? | x * 33000, SDS-PAGE | Burkholderia cepacia |
| ? | x * 19400, SDS-PAGE | Bacillus subtilis |
| ? | x * 48000, recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Bacillus subtilis |
| lipase | - |
Pseudomonas fluorescens |
| lipase | - |
Pseudomonas aeruginosa |
| lipase | - |
Pseudomonas alcaligenes |
| lipase | - |
Burkholderia cepacia |
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