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Literature summary for 3.1.1.29 extracted from
- Vms1 and ANKZF1 peptidyl-tRNA hydrolases release nascent chains from stalled ribosomes (2018), Nature, 557, 446-451 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ANKZF1, transient recombinant expression of His-tagged wild-type and mutant enzymes in HEK-293 cells | Homo sapiens |
| gene VMS1, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Q246L | site-directed mutagenesis, inactive active site mutant | Homo sapiens |
| Q295L | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptidyl-tRNA + H2O | Saccharomyces cerevisiae | - |
peptide + tRNA | - |
? |  |
| peptidyl-tRNA + H2O | Homo sapiens | - |
peptide + tRNA | - |
? | |
| peptidyl-tRNA + H2O | Saccharomyces cerevisiae ATCC 204508 | - |
peptide + tRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9H8Y5 | - |
- |
| Saccharomyces cerevisiae | Q04311 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 | Q04311 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography | Saccharomyces cerevisiae |
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Flag-tagged CRPNSKn-tRNA + H2O | - |
Saccharomyces cerevisiae | Flag-tagged CRPNSKn + tRNA | - |
? | |
| Flag-tagged CRPNSKn-tRNA + H2O | purified recombinant Ankzf1 catalyzes deacylation | Homo sapiens | Flag-tagged CRPNSKn + tRNA | - |
? | |
| Flag-tagged CRPNSKn-tRNA + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | Flag-tagged CRPNSKn + tRNA | - |
? | |
| peptidyl-tRNA + H2O | - |
Saccharomyces cerevisiae | peptide + tRNA | - |
? | |
| peptidyl-tRNA + H2O | - |
Homo sapiens | peptide + tRNA | - |
? | |
| peptidyl-tRNA + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | peptide + tRNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ankzf1 | - |
Homo sapiens |
| Cdc48 adaptor | - |
Saccharomyces cerevisiae |
| peptidyl-tRNA hydrolase | - |
Saccharomyces cerevisiae |
| peptidyl-tRNA hydrolase | - |
Homo sapiens |
| Vms1 | - |
Saccharomyces cerevisiae |
| ZNF744 | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | Ankzf1 is Saccharomyces cerevisiae Vms1 homologue | Homo sapiens |
| evolution | Vms1 is a member of a clade of eRF1 homologues that is designated the Vms1-like RF1 clade (VLRF1) | Saccharomyces cerevisiae |
| metabolism | enzyme Vms1, which contains a Cdc48-binding VIM motif, is implicated in the ribosome quality control (RQC) pathway. The 60S subunits retain the peptidyl-tRNA nascent chains, which recruit the RQC complex comprised of Rqc1-Rqc2-Ltn1-Cdc48-Ufd1-Npl4. Nascent chains ubiquitylated by the E3/ubiquitin ligase Ltn1 are extracted from the 60S by the ATPase Cdc48-Ufd1-Npl4 and presented to the 26S proteasome for degradation. The Vms1-dependent reaction does not display strong dependence on substrate ubiquitylation | Saccharomyces cerevisiae |
| additional information | enzyme Vms1 activity is dependent on a conserved catalytic glutamine. The putative catalytic region of Vms1 and other VLRF1 proteins is flanked by additional N- and C-terminal domains. The N-terminal C2H2-zinc finger of Vms1 is specifically related to those of Rei1 and certain SBDS paralogues, which function at late steps in 60S subunit maturation | Saccharomyces cerevisiae |
| physiological function | Ankzf1 is a peptidyl-tRNA hydrolase | Homo sapiens |
| physiological function | the Cdc48 adaptor Vms1 is a peptidyl-tRNA hydrolase. Yeast Cdc48, a AAA+ ATPase that is conserved across eukaryotes and archaea, is a protein unfoldase that engages in myriad cellular functions through binding of adaptors such as Ufd1-Npl4 (UN), which bind both Cdc48 and ubiquitylated substrate proteins | Saccharomyces cerevisiae |
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