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Literature summary for 3.1.1.29 extracted from

  • Kabra, A.; Fatma, F.; Shahid, S.; Pathak, P.P.; Yadav, R.; Pulavarti, S.V.; Tripathi, S.; Jain, A.; Arora, A.
    Structural characterization of peptidyl-tRNA hydrolase from Mycobacterium smegmatis by NMR spectroscopy (2016), Biochim. Biophys. Acta, 1864, 1304-1314 .
    View publication on PubMed
Search for more literature for 3.1.1.29

Cloned(Commentary)

Cloned (Comment) Organism
gne pth, recombinant expression of N-terminally His-tagged enzyme Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidyl-tRNA + H2O Mycolicibacterium smegmatis
-
 peptide + tRNA
-
 ?
peptidyl-tRNA + H2O Mycolicibacterium smegmatis ATCC 700084
-
 peptide + tRNA
-
 ?
peptidyl-tRNA + H2O Mycolicibacterium smegmatis mc(2)155
-
 peptide + tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0R3D3
-
-
Mycolicibacterium smegmatis ATCC 700084 A0R3D3
-
-
Mycolicibacterium smegmatis mc(2)155 A0R3D3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptidyl-tRNA + H2O
-
 Mycolicibacterium smegmatis peptide + tRNA
-
 ?
peptidyl-tRNA + H2O
-
 Mycolicibacterium smegmatis ATCC 700084 peptide + tRNA
-
 ?
peptidyl-tRNA + H2O
-
 Mycolicibacterium smegmatis mc(2)155 peptide + tRNA
-
 ?

Subunits

Subunits Comment Organism
monomer analysis and comparisons of the overall NMR solution structure of enzyme MsPth, secondary structure comparisons. In solution, enzyme MsPth exists as a monomeric protein consisting of an alpha/beta globular domain with seven beta-strands and six alpha-helices Mycolicibacterium smegmatis

Synonyms

Synonyms Comment Organism
MsPth
-
 Mycolicibacterium smegmatis
peptidyl-tRNA hydrolase
-
 Mycolicibacterium smegmatis
PTH
-
 Mycolicibacterium smegmatis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
61.71
-
 melting temperature of enzyme MsPth Mycolicibacterium smegmatis

General Information

General Information Comment Organism
additional information enzyme structure and dynamics by NMR spectroscopy and molecular dynamics simulations. Of the substrate binding segments, the gate loop is rigid, the base loop displays slow motions, while the lid loop displays fast timescale motions. The NMR structure of Mycobacterium smegmatis MsPth shares the canonical Pth fold with theNMR structure of Mycobacterium tuberculosis MtPth Mycolicibacterium smegmatis