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Literature summary for 3.1.1.29 extracted from

  • Giorgi, L.; Plateau, P.; O'Mahony, G.; Aubard, C.; Fromant, M.; Thureau, A.; Grotli, M.; Blanquet, S.; Bontems, F.
    NMR-based substrate analog docking to Escherichia coli peptidyl-tRNA hydrolase (2011), J. Mol. Biol., 412, 619-633.
    View publication on PubMed
Search for more literature for 3.1.1.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain K37DELTArecAlambdaDE3 Escherichia coli

Protein Variants

Protein Variants Comment Organism
H20A the mutant is unable to hydrolyze 2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine Escherichia coli
N10A the mutant shows strongly reduced activity with diacetyl-lysyl-tRNALys and L-Lys-tRNALys compared to the wild type enzyme Escherichia coli
N10D the mutant shows strongly reduced activity with diacetyl-lysyl-tRNALys and L-Lys-tRNALys compared to the wild type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
3'-L-(N,N-diacetyl-lysinyl)amino-3'-deoxyadenosine
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A7D1
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine + H2O minimalist substrate Escherichia coli ?
-
 ?
diacetyl-lysyl-tRNALys + H2O diacetyl-lysyl-tRNALys is hydrolyzed by the wild type enzyme 360fold more efficiently than Lys-tRNALys Escherichia coli diacetyl-lysine + tRNALys
-
 ?
L-Lys-tRNALys + H2O
-
 Escherichia coli L-lysine + tRNALys
-
 ?

Synonyms

Synonyms Comment Organism
peptidyl-tRNA hydrolase
-
 Escherichia coli
PTH
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
12
-
 3'-L-(N,N-diacetyl-lysinyl)amino-3'-deoxyadenosine at pH 6.0, with 50 mM sodium acetate and 200 mM NaCl, at 28°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.51
-
 2'(3')-O-L-(N,N-diacetyl-lysinyl)adenosine wild type enzyme, 28°C, 20 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM dithiothreitol Escherichia coli