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Literature summary for 3.1.1.29 extracted from

  • Fromant, M.; Scvhmitt, E.; Mechulam, Y.; Lazennec, C.; Plateau, P.; Blanquet, S.
    Crystal structure at 1.8 A resolution and identification of active site residues of Sulfolobus solfataricus peptidyl-tRNA hydrolase (2005), Biochemistry, 44, 4292-4301.
No PubMed abstract available
Search for more literature for 3.1.1.29

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, 1.8 A resolution Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
D86A kcat/Km for diacetyl-lysyl-tRNALys is 0.22% of wild-type value Saccharolobus solfataricus
D86A/K18A kcat/Km for diacetyl-lysyl-tRNALys is 0.17% of wild-type value Saccharolobus solfataricus
H25A kcat/Km for diacetyl-lysyl-tRNALys is 13% of wild-type value Saccharolobus solfataricus
K118A kcat/Km for diacetyl-lysyl-tRNALys is 87% of wild-type value Saccharolobus solfataricus
K18A kcat/Km for diacetyl-lysyl-tRNALys is 0.2% of wild-type value Saccharolobus solfataricus
K56A kcat/Km for diacetyl-lysyl-tRNALys is 12% of wild-type value Saccharolobus solfataricus
Q22A kcat/Km for diacetyl-lysyl-tRNALys is 20% of wild-type value Saccharolobus solfataricus
Q54A kcat/Km for diacetyl-lysyl-tRNALys is 47% of wild-type value Saccharolobus solfataricus
T90A kcat/Km for diacetyl-lysyl-tRNALys is 1.4% of wild-type value Saccharolobus solfataricus
T98A kcat/Km for diacetyl-lysyl-tRNALys is 34% of wild-type value Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme T98A Saccharolobus solfataricus
1.6
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme Q22A Saccharolobus solfataricus
2.9
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme Q54A Saccharolobus solfataricus
3.2
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K56A Saccharolobus solfataricus
4.4
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme H25A Saccharolobus solfataricus
5.3
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K18A Saccharolobus solfataricus
5.4
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme D86A Saccharolobus solfataricus
6.7
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme D86A/K18A Saccharolobus solfataricus
7.2
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme T90A Saccharolobus solfataricus
9.1
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, wild-type enzyme Saccharolobus solfataricus
12.8
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K118A Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diacetyl-lysyl-tRNALys
-
 Saccharolobus solfataricus diacetyl-Lys + tRNALys
-
 ?

Synonyms

Synonyms Comment Organism
YbaK
-
 Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme D86A/K18A Saccharolobus solfataricus
0.002
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K18A Saccharolobus solfataricus
0.0025
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme D86A Saccharolobus solfataricus
0.02
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme T90A Saccharolobus solfataricus
0.06
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme Q22A Saccharolobus solfataricus
0.06
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme T98A Saccharolobus solfataricus
0.07
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K56A Saccharolobus solfataricus
0.1
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme H25A Saccharolobus solfataricus
0.27
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme Q54A Saccharolobus solfataricus
1.8
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, wild-type enzyme Saccharolobus solfataricus
2.2
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, mutant enzyme K118A Saccharolobus solfataricus