Literature summary for 3.1.1.29 extracted from

Bonin, P.D.; Choi, G.H.; Trepod, C.M.; Mott, J.E.; Lyle, S.B.; Cialdella, J.I.; Sarver, R.W.; Marshall, V.P.; Erickson, L.A.
Expression, purification, and characterization of peptidyl-tRNA hydrolase from Staphylococcus aureus (2002), Protein Expr. Purif., 24, 123-130.

Search for more literature for 3.1.1.29

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0028	-	diacetyl-lysine-tRNA	23-24°C, pH 7.2	Staphylococcus aureus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	maximal activity at 5 mM	Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23000	-	x * 23000, SDS-PAGE	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-substituted aminoacyl-tRNA + H2O	Staphylococcus aureus	-	N-substituted amino acid + tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	Q6YP15	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged Pth	Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.07	-	-	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diacetyl-lysine-tRNA + H2O	-	Staphylococcus aureus	diacetyl-lysine + tRNA	-	?
N-substituted aminoacyl-tRNA + H2O	-	Staphylococcus aureus	N-substituted amino acid + tRNA	-	?

Subunits

Subunits	Comment	Organism
?	x * 23000, SDS-PAGE	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
peptidyl-tRNA hydrolase	-	Staphylococcus aureus
PTH	-	Staphylococcus aureus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Staphylococcus aureus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8.5	approx. 38% at pH 6.0, approx. 20% of maximal activity at pH 8.5	Staphylococcus aureus

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Release 2023.1 (January 2023)