Literature summary for 3.1.1.27 extracted from

Kobayashi, J.; Yoshikane, Y.; Yagi, T.; Baba, S.; Mizutani, K.; Takahashi, N.; Mikami, B.
Structure of 4-pyridoxolactonase from Mesorhizobium loti (2014), Acta Crystallogr. Sect. F, 70, 424-432.

Search for more literature for 3.1.1.27

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli B834 (DE3) cells	Mesorhizobium loti

Crystallization (Commentary)

Crystallization (Comment)	Organism
native and SeMet-labelled protein in complex with 5-pyridoxolactone, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 50 mM ammonium citrate, pH 5.1	Mesorhizobium loti

Inhibitors

Inhibitors	Comment	Organism	Structure
5-Pyridoxolactone	competitve inhibitor	Mesorhizobium loti

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	dependent on	Mesorhizobium loti

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-pyridoxolactone + H2O	Mesorhizobium loti	-	4-pyridoxate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mesorhizobium loti	Q988B9	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography, hydroxyapatite column chromatography, and Shodex protein KW-803 gel filtration	Mesorhizobium loti

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-pyridoxolactone + H2O	-	Mesorhizobium loti	4-pyridoxate	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Mesorhizobium loti

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)