Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli BL21 (DE3) cells harbouring plasmid pET21a6805 | Mesorhizobium loti |
Crystallization (Comment) | Organism |
---|---|
by the sitting-drop vapour-diffusion method, at 4°C. Free enzyme to 2.0 A resolution, belongs to the monoclinic space group C2, with unit-cell parameters a = 77.93, b = 38.88, c = 81.60 A, beta = 117.33. The 5-pyridoxolactone-bound enzyme to 1.9 A resolution, belongs to the monoclinic space group C2, with unit-cell parameters a = 86.24, b = 39.35, c = 82.68 A, beta = 118.02 | Mesorhizobium loti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Mesorhizobium loti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium loti | - |
- |
- |
Mesorhizobium loti MAFF303099 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme purified sequentially by gel filtration and ammonium sulfate precipitation | Mesorhizobium loti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-pyridoxolactone + H2O | - |
Mesorhizobium loti | 4-pyridoxate | - |
? | |
4-pyridoxolactone + H2O | - |
Mesorhizobium loti MAFF303099 | 4-pyridoxate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Mesorhizobium loti |
Synonyms | Comment | Organism |
---|---|---|
4-pyridoxolactonase | - |
Mesorhizobium loti |