Literature summary for 3.1.1.27 extracted from

Funami, J.; Yoshikane, Y.; Kobayashi, H.; Yokochi, N.; Yuan, B.; Iwasaki, K.; Ohnishi, K.; Yagi, T.
4-Pyridoxolactonase from a symbiotic nitrogen-fixing bacterium Mesorhizobium loti: cloning, expression, and characterization (2005), Biochim. Biophys. Acta, 1753, 234-239.

Search for more literature for 3.1.1.27

Cloned(Commentary)

Cloned (Comment)	Organism
-	Mesorhizobium loti

General Stability

General Stability	Organism
enzyme shows very low activity in Tris-HCl bufffers	Mesorhizobium loti

Inhibitors

Inhibitors	Comment	Organism	Structure
5-Pyridoxolactone	competitive	Mesorhizobium loti
Hg2+	0.1 mM, complete inhibition	Mesorhizobium loti

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.008	-	4-Pyridoxolactone	pH 7.5	Mesorhizobium loti
0.319	-	4-pyridoxate	pH 7.5	Mesorhizobium loti

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	one mole per mole of subunit, tightly bound to the protein	Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29855	-	2 * 31000, SDS-PAGE, 2 * 29855, calculated	Mesorhizobium loti
31000	-	2 * 31000, SDS-PAGE, 2 * 29855, calculated	Mesorhizobium loti
59000	-	gel filtration	Mesorhizobium loti

Organism

Organism	UniProt	Comment	Textmining
Mesorhizobium loti	-	overexpression in Escherichia coli	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-pyridoxate	-	Mesorhizobium loti	4-pyridoxolactone + H2O	-	r
4-pyridoxolactone + H2O	-	Mesorhizobium loti	4-pyridoxate	-	r
N-hexanoyl-D,L-homoserine lactone + H2O	41% of the activity with 4-pyridoxolactone	Mesorhizobium loti	N-hexanoyl-D,L-homoserine	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 31000, SDS-PAGE, 2 * 29855, calculated	Mesorhizobium loti
More	N-terminal amino acid sequence	Mesorhizobium loti

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	70	-	Mesorhizobium loti

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	10 min, stable	Mesorhizobium loti
55	-	10 min, 64% residual activity	Mesorhizobium loti
60	-	10 min, 14% residual activity	Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.4	-	4-pyridoxate	pH 7.5	Mesorhizobium loti
12.2	-	4-Pyridoxolactone	pH 7.5	Mesorhizobium loti

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Mesorhizobium loti

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	-	36% of maximum activity	Mesorhizobium loti
9.5	-	10% of maximum activity	Mesorhizobium loti

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.15	-	5-Pyridoxolactone	pH 7.5	Mesorhizobium loti

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Release 2023.1 (January 2023)