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Literature summary for 3.1.1.26 extracted from

  • Matsuda, H.; Hirayama, O.
    Enzymatic properties of a galactolipase from rice bran (1979), Agric. Biol. Chem., 43, 697-703.
No PubMed abstract available
Search for more literature for 3.1.1.26

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol traces of activity remain with 10 mM at pH 7.5 Oryza sativa
CaCl2 79% inhibition at a concentration of 10 mM Oryza sativa
cetyltrimethylammonium chloride 65% inhibition at 1 mM Oryza sativa
cysteine traces of activity remain with 100 mM at pH 7.5 Oryza sativa
diisopropylfluorophosphate 7% remaining activity with 1 mM at pH 7.5 Oryza sativa
EDTA no inhibition at 1 mM, 78% inhibition at 10 mM Oryza sativa
additional information not affected by 0.3 mM p-chloromercuribenzoate, and 30 mM N-ethylmaleimide Oryza sativa
SDS 0.1% complete inhibition Oryza sativa
Triton X-100 31% inhibition at 0.1%, 83% inhibition at 1% Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 monogalactosyldiacylglycerol
-
 Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 124% activity compared to assay without additives at 0.1 mM Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
digalactosyldiacylglycerol + H2O Oryza sativa
-
 ?
-
 ?
monogalactosyldiacylglycerol + H2O Oryza sativa
-
 ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone complete inhibition at 1% Oryza sativa
Ethanol complete inhibition at 1% Oryza sativa
ether complete inhibition at 1% Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
digalactosyldiacylglycerol + H2O 169% of the activity with monogalactosyldiacylglycerol Oryza sativa digalactosylglycerol + fatty acid
-
 ?
digalactosyldiacylglycerol + H2O
-
 Oryza sativa ?
-
 ?
lysophosphatidylcholine + H2O 33% of the activity with monogalactosyldiacylglycerol Oryza sativa ?
-
 ?
monogalactosyldiacylglycerol + H2O
-
 Oryza sativa galactosylglycerol + fatty acid
-
 ?
monogalactosyldiacylglycerol + H2O
-
 Oryza sativa ?
-
 ?
monogalactosylmonoacylglycerol + H2O 111% of the activity with monogalactosyldiacylglycerol Oryza sativa monogalactosylglycerol + fatty acid
-
 ?
phosphatidylcholine + H2O 27% of the activity with monogalactosyldiacylglycerol Oryza sativa ?
-
 ?
phosphatidylethanolamine + H2O traces of the activity with monogalactosyldiacylglacerol Oryza sativa ?
-
 ?
triacylglycerol + H2O traces of the activity with monogalactosyldiacylglycerol Oryza sativa ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Oryza sativa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 45 0°C: 50% of the maximal activity, 45°C: 40% of the maximal activity Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 incubation for 10 min at pH 7.5, 95% loss of activity, retains more thant 60% of its activity at pH 5.5 Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 Tris-HCl buffer Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
4 9 pH 4: 25% of the maximal activity, pH 9: 50% of the maximal activity Oryza sativa