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Literature summary for 3.1.1.26 extracted from

  • Eydoux, C.; Spinelli, S.; Davis, T.L.; Walker, J.R.; Seitova, A.; Dhe-Paganon, S.; De Caro, A.; Cambillau, C.; Carriere, F.
    Structure of human pancreatic lipase-related protein 2 with the lid in an open conformation (2008), Biochemistry, 47, 9553-9564.
    View publication on PubMed
Search for more literature for 3.1.1.26

Activating Compound

Activating Compound Comment Organism Structure
additional information using the partly soluble short chain tributyrin, the activity of both HPLRP2 N336Q mutant and HPLRP2 is high in the presence of bile salts and in the absence of colipase Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in SF9 insect cells (wild type enzyme) and in Pichia pastoris (mutant enzyme N336Q) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, using 1.17 M (NH4)2SO4, 0.1 M Na-cacodylate pH 5.5, 0.04 M NaCl for wild type enzyme, and 2.05 M ammonium sulfate, 0.1 M HEPES, pH 6.7 for mutant enzyme N336Q Homo sapiens

Protein Variants

Protein Variants Comment Organism
N336Q the mutant enzyme is not glycosylated and shows identical kinetic properties to the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
E600 in the absence of bile salts and colipase, the time required to obtain 50% inactivation of PLRP2 by E600 is 3 min, in the presence of 4 mM sodium taurodeoxycholate and colipase, the rate of inhibition of PLRP2 by E600 increases significantly and 50% inhibition is reached within 1 min Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50080
-
 mature PLRP2, calculated from amino acid sequence Homo sapiens
52820
-
 glycosylated enzyme, LC-ESI-TOF mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens acts preferentially on substrates forming monomers or small aggregates (micelles) dispersed in solution like monoglycerides, phospholipids and galactolipids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P54317
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q HP column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
 wild type enzyme, using 1,2(2,3)-diolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
3.4
-
 mutant enzyme N336Q, using 1,2(2,3)-diolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
4.3
-
 mutant enzyme N336Q, using triolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
5
-
 wild type enzyme, using triolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
40.2
-
 mutant enzyme N336Q, using trioctanoin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
43
-
 wild type enzyme, using trioctanoin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
212
-
 mutant enzyme N336Q, using tributyrin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
247
-
 mutant enzyme N336Q, using 1(3)-monoolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
287
-
 wild type enzyme, using tributyrin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
317
-
 wild type enzyme, using 1(3)-monoolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1(3)-monoolein + H2O
-
 Homo sapiens ?
-
 ?
1,2(2,3)-diolein + H2O
-
 Homo sapiens ?
-
 ?
1,2-di-O-dodecanoyl-3-O-beta-D-galactopyranosyl-sn-glycerol + H2O galactolipase activity of LRP2 Homo sapiens 2,3-dihydroxypropyl beta-D-galactopyranoside + dodecanoate
-
 ?
3-O-(6-O-alpha-D-galactopyranosyl-beta-D-galactopyranosyl)-1,2-di-O-dodecanoyl-sn-glycerol + H2O galactolipase activity of LRP2 Homo sapiens 2,3-dihydroxypropyl 6-O-beta-D-galactopyranosyl-beta-D-galactopyranoside + 2 dodecanoate
-
 ?
digalactosyldiacylglycerol + H2O
-
 Homo sapiens digalactosylglycerol + fatty acid
-
 ?
L-alpha-phosphatidylcholine + H2O phospholipase activity of LRP2 Homo sapiens ?
-
 ?
monogalactosyldiacylglycerol + H2O
-
 Homo sapiens galactosylglycerol + fatty acid
-
 ?
additional information acts preferentially on substrates forming monomers or small aggregates (micelles) dispersed in solution like monoglycerides, phospholipids and galactolipids Homo sapiens ?
-
 ?
tributyrin + H2O
-
 Homo sapiens ?
-
 ?
trioctanoin + H2O
-
 Homo sapiens ?
-
 ?
triolein + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
pancreatic lipase-related protein 2
-
 Homo sapiens
PLRP2
-
 Homo sapiens