Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Origami B (DE3) cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.316 | - |
(R)-gamma-valerolactone | at pH 7.0 and 30°C | Homo sapiens | |
36.8 | - |
gamma-butyrolactone | at pH 7.0 and 30°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 4.5 mM used in assay conditions | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood serum | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-gamma-valerolactone + H2O | - |
Homo sapiens | 4-hydroxypentanoic acid | - |
? | |
gamma-butyrolactone + H2O | - |
Homo sapiens | 4-hydroxybutanoate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36.621 | - |
(R)-gamma-valerolactone | at pH 7.0 and 30°C | Homo sapiens | |
147.209 | - |
gamma-butyrolactone | at pH 7.0 and 30°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
gamma-butyrolactone | at pH 7.0 and 30°C | Homo sapiens | |
27.82 | - |
(R)-gamma-valerolactone | at pH 7.0 and 30°C | Homo sapiens |