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Literature summary for 3.1.1.25 extracted from

  • Hiblot, J.; Bzdrenga, J.; Champion, C.; Chabriere, E.; Elias, M.
    Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia (2015), Sci. Rep., 5, 8372.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.25

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method at 20°C, X-ray structures of the enzyme bound to a fatty acid and to its substrate acyl-homoserine lactone Vulcanisaeta moutnovskia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 gamma-dodecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.047
-
 gamma-nonalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.105
-
 delta-undecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.12
-
 gamma-undecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.186
-
 3-oxo-octanoyl-L-homoserine lactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.231
-
 3-oxo-decanoyl-L-homoserine lactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.262
-
 octanoyl-L-homoserine lactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.839
-
 delta-dodecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.872
-
 gamma-heptalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
1.22
-
 delta-nonalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
3.7
-
 Gamma-caprolactone pH 8.3, 40°C Vulcanisaeta moutnovskia

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the enzyme possesses a bi-cobalt active site Vulcanisaeta moutnovskia

Organism

Organism UniProt Comment Textmining
Vulcanisaeta moutnovskia F0QXN6
-
-
Vulcanisaeta moutnovskia 768-28 F0QXN6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vulcanisaeta moutnovskia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxo-decanoyl-L-homoserine lactone + H2O
-
 Vulcanisaeta moutnovskia N-(3-oxodecanoyl)-L-homoserine
-
 ?
3-oxo-decanoyl-L-homoserine lactone + H2O
-
 Vulcanisaeta moutnovskia 768-28 N-(3-oxodecanoyl)-L-homoserine
-
 ?
3-oxo-octanoyl-L-homoserine lactone + H2O
-
 Vulcanisaeta moutnovskia N-(3-oxooctanoyl)-L-homoserine
-
 ?
delta-dodecalactone + H2O
-
 Vulcanisaeta moutnovskia 5-hydroxydecanoate
-
 ?
delta-nonalactone + H2O
-
 Vulcanisaeta moutnovskia 5-hydroxynonanoate
-
 ?
delta-nonalactone + H2O
-
 Vulcanisaeta moutnovskia 768-28 5-hydroxynonanoate
-
 ?
delta-undecalactone + H2O
-
 Vulcanisaeta moutnovskia 5-hydroxydecanoate
-
 ?
gamma-caprolactone + H2O
-
 Vulcanisaeta moutnovskia 4-hydroxyhexanoic acid
-
 ?
gamma-caprolactone + H2O
-
 Vulcanisaeta moutnovskia 768-28 4-hydroxyhexanoic acid
-
 ?
gamma-dodecalactone + H2O
-
 Vulcanisaeta moutnovskia 4-hydroxydodecanoate
-
 ?
gamma-heptalactone + H2O
-
 Vulcanisaeta moutnovskia 4-hydroxyheptanoate
-
 ?
gamma-nonalactone + H2O
-
 Vulcanisaeta moutnovskia 4-hydroxynonanoate
-
 ?
gamma-nonalactone + H2O
-
 Vulcanisaeta moutnovskia 768-28 4-hydroxynonanoate
-
 ?
gamma-undecalactone + H2O
-
 Vulcanisaeta moutnovskia 4-hydroxyundecanoate
-
 ?
additional information no activity with dihydrocoumarin Vulcanisaeta moutnovskia ?
-
 ?
additional information no activity with dihydrocoumarin Vulcanisaeta moutnovskia 768-28 ?
-
 ?
octanoyl-L-homoserine lactone + H2O
-
 Vulcanisaeta moutnovskia N-octanoyl-L-homoserine
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Vulcanisaeta moutnovskia

Synonyms

Synonyms Comment Organism
VmoLac
-
 Vulcanisaeta moutnovskia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Vulcanisaeta moutnovskia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
128
-
 Tm-value Vulcanisaeta moutnovskia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 gamma-dodecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.35
-
 3-oxo-octanoyl-L-homoserine lactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.47
-
 3-oxo-decanoyl-L-homoserine lactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.59
-
 octanoyl-L-homoserine lactone pH 8.3, 40°C Vulcanisaeta moutnovskia
8.86
-
 gamma-undecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
27.25
-
 gamma-heptalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
28.08
-
 delta-dodecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
44.49
-
 gamma-nonalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
60.78
-
 delta-undecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
88.91
-
 delta-nonalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
112.3
-
 Gamma-caprolactone pH 8.3, 40°C Vulcanisaeta moutnovskia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
 assay at Vulcanisaeta moutnovskia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
 Gamma-caprolactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.031
-
 gamma-heptalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.0335
-
 delta-dodecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.058
-
 delta-undecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.073
-
 delta-nonalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.074
-
 gamma-undecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.078
-
 gamma-dodecalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
0.948
-
 gamma-nonalactone pH 8.3, 40°C Vulcanisaeta moutnovskia
1.88
-
 3-oxo-octanoyl-L-homoserine lactone pH 8.3, 40°C Vulcanisaeta moutnovskia
2.05
-
 3-oxo-decanoyl-L-homoserine lactone pH 8.3, 40°C Vulcanisaeta moutnovskia
2.23
-
 octanoyl-L-homoserine lactone pH 8.3, 40°C Vulcanisaeta moutnovskia