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Literature summary for 3.1.1.25 extracted from

  • Porzio, E.; Di, Gennaro, S.; Palma, A.; Manco, G.
    Mn2+ modulates the kinetic properties of an archaeal member of the PLL family (2013), Chem. Biol. Interact., 203, 251-256.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
SacPox is expressed in the presence of 0.2 mM of each of the following salts: CdCl2, CoCl2, MnCl2, ZnCl2, NiSO4. The gene sacpox is cloned in the expression vector pT7-7, and overexpressed in Escherichia coli BL21(DE3) cells. The highest activity is obtained for the crude extract of a Mn2+-enriched culture Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus acidocaldarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
549
-
substrate: 5-thiobutyl-butyro-1,4-lactone, pH 8.5, 70°C, Mn2+-containing enzyme Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-thiobutyl-butyro-1,4-lactone + H2O
-
Sulfolobus acidocaldarius ?
-
?

Synonyms

Synonyms Comment Organism
SacPox
-
Sulfolobus acidocaldarius