Application | Comment | Organism |
---|---|---|
synthesis | the recombinant enzyme is a highly efficient biocatalyst for asymmetric synthesis of chiral compounds | Fusarium proliferatum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109(DE3) cells | Fusarium proliferatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 33.2% residual activity at 1 mM | Fusarium proliferatum | |
Al3+ | 22.4% residual activity at 1 mM | Fusarium proliferatum | |
Co2+ | 10.7% residual activity at 1 mM | Fusarium proliferatum | |
Cu+ | 13.6% residual activity at 1 mM | Fusarium proliferatum | |
Cu2+ | 11.2% residual activity at 1 mM | Fusarium proliferatum | |
Fe2+ | 15.6% residual activity at 1 mM | Fusarium proliferatum | |
Fe3+ | 3.3% residual activity at 1 mM | Fusarium proliferatum | |
Hg2+ | 2.1% residual activity at 1 mM | Fusarium proliferatum | |
Li+ | 24.7% residual activity at 1 mM | Fusarium proliferatum | |
Mg2+ | 32.4% residual activity at 1 mM | Fusarium proliferatum | |
Mn2+ | 22.7% residual activity at 1 mM | Fusarium proliferatum | |
additional information | EDTA does not exhibit significant effect on enzyme activity | Fusarium proliferatum | |
Ni2+ | 55.4% residual activity at 1 mM | Fusarium proliferatum | |
Pb2+ | 13.9% residual activity at 1 mM | Fusarium proliferatum | |
Zn2+ | 14.3% residual activity at 1 mM | Fusarium proliferatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
121 | - |
DL-pantoyllactone | recombinant enzyme, at pH 6.7-6.9, 30°C | Fusarium proliferatum | |
290.2 | - |
2-hydroxybutyrolactone | recombinant enzyme, at pH 6.7-6.9, 30°C | Fusarium proliferatum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 7.4% activation at 1 mM. No significant effect of Ca2+ on the stability of recombinant enzyme is observed when incubated together for 60 h at 30°C or 4°C | Fusarium proliferatum | |
additional information | the enzyme is not affected by Na+ and K+ | Fusarium proliferatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, recombinant protein, SDS-PAGE | Fusarium proliferatum |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 49% residual activity at 10% (v/v), when the concentration of methanol is increased up to 20% (v/v), up to 30% of the initial activity is retained | Fusarium proliferatum |
acetonitrile | 55% residual activity at 10% (v/v), when the concentration of methanol is increased up to 20% (v/v), up to 30% of the initial activity is retained | Fusarium proliferatum |
dimethylformamide | 51% residual activity at 10% (v/v) | Fusarium proliferatum |
Ethanol | 55% residual activity at 10% (v/v) | Fusarium proliferatum |
Methanol | 81% residual activity at 10% (v/v), when the concentration of methanol is increased up to 20% (v/v), 60.5% of the initial activity is retained | Fusarium proliferatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium proliferatum | B2LYJ5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Fusarium proliferatum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
341.2 | - |
recombinant enzyme after 14.4fold purification, using DL-pantoyl lactone as substrate, at pH 6.7-6.9, 30°C | Fusarium proliferatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-bromobutyrolactone + H2O | less than 1% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
2-hydroxybutyrolactone + H2O | 2450% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
4-butyrolactone + H2O | less than 1% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
4-phenyl-2-hydroxyl-4-butyrolactone + H2O | 22% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
DL-pantoyllactone + H2O | 100% activity | Fusarium proliferatum | pantoic acid | - |
? | |
ethyl acetate + H2O | less than 1% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
methyl 2-chloroacetate + H2O | 11.6% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
methyl 2-chloropropanoate + H2O | 2.3% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
methyl lactate + H2O | less than 1% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
methyl mandelate + H2O | less than 1% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
additional information | no activity with 4-nitrophenyl acetate and 4-nitrophenyl butyrate | Fusarium proliferatum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, recombinant protein, SDS-PAGE | Fusarium proliferatum |
Synonyms | Comment | Organism |
---|---|---|
levo-lactonase | - |
Fusarium proliferatum |
Organism | Comment | Expression |
---|---|---|
Fusarium proliferatum | by increasing the biomass concentration and lowering the inducer (isopropyl-beta-D-thiogalactoside) concentration and induction temperature, the recombinant lactonase production in Escherichia coli is significantly enhanced (20times higher than that of wild type strain Fusarium proliferatum ECU2002) | up |