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Literature summary for 3.1.1.25 extracted from

  • Renault, F.; Carus, T.; Clery-Barraud, C.; Elias, M.; Chabriere, E.; Masson, P.; Rochu, D.
    Integrative analytical approach by capillary electrophoresis and kinetics under high pressure optimized for deciphering intrinsic and extrinsic cofactors that modulate activity and stability of human paraoxonase (PON1) (2010), J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 878, 1346-1355.
    View publication on PubMed
Search for more literature for 3.1.1.25

Activating Compound

Activating Compound Comment Organism Structure
additional information binding of human phosphate binding protein amends the size of the oligomeric states and exerts a stabilizing effect on the activity Homo sapiens
phosphate
-
 synthetic construct
phosphate maintains activity, modulates oligomeric state Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
bacterially expressed chimeric G2E6 synthetic construct

Crystallization (Commentary)

Crystallization (Comment) Organism
phosphate ion is bound to the catalytic calcium in the structure of crystallized recombinant G2E6 synthetic construct

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxyquinoline
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 synthetic construct
Ca2+ is essential for activity and stability of lactonase, modulates oligomeric state Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
synthetic construct
-
 chimeric mammalian dubbed G3C9-8His, obtained using directed evolution via gene shuffling of human, rabbit, rat and mouse genes
-

Purification (Commentary)

Purification (Comment) Organism
by pseudo-affinity chromatography and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris buffer, 50 mM NaCl, 1 mM CaCl2, 0.1% tergitol, pH 8.0, 1 month Homo sapiens
4°C, 50 mM Tris buffer, 50 mM NaCl, 1 mM CaCl2, 0.1% tergitol, pH 8.0, 1 month synthetic construct

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-coumaranone + H2O
-
 Homo sapiens (2-hydroxyphenyl)acetic acid
-
 ?
2-coumaranone + H2O
-
 synthetic construct (2-hydroxyphenyl)acetic acid
-
 ?

Synonyms

Synonyms Comment Organism
lactonase
-
 Homo sapiens
lactonase
-
 synthetic construct

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
 2-coumaranone lactonase/human phosphate binding protein (9:1), in 50 mM Tris buffer, 1 mM NaCl, pH 8.0 at 25°C Homo sapiens
73.3
-
 2-coumaranone lactonase/human phosphate binding protein (1:1), in 50 mM Tris buffer, 1 mM NaCl, pH 8.0 at 25°C Homo sapiens
131.6
-
 2-coumaranone recombinant lactonase, in 50 mM Tris buffer, 1 mM NaCl, pH 8.0 at 25°C synthetic construct