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Literature summary for 3.1.1.25 extracted from

  • Hu, X.; Jiang, X.; Lenz, D.E.; Cerasoli, D.M.; Wallqvist, A.
    In silico analyses of substrate interactions with human serum paraoxonase 1 (2009), Proteins, 75, 486-498.
    View publication on PubMed
Search for more literature for 3.1.1.25

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D183N the mutant disfavors paraoxon binding due to its charged nature and possible electrostatic repulsion with the phosphate group of paraoxon Homo sapiens
F222D the mutation abolishes both esterase and PON activity of PON1 Homo sapiens
F222Y the mutation abolishes the PON activity of PON1 but retains the esterase activity with a 1.5fold increase in KM for phenyl acetate Homo sapiens
H115W the mutant shows a 2fold increase in PON1 activity compared to the wild type enzyme Homo sapiens
L69V the mutant shows a 4-16fold increase in PON1 activity compared to the wild type enzyme Homo sapiens
L69V/V369A the mutant shows increased paraoxon binding affinity compared to the wild type enzyme Homo sapiens
R192Q the polymorphic residue R192Q interacts with the leaving group of paraoxon, suggesting it plays an important role in the proper positioning of this substrate in the active site Homo sapiens
S193P the mutation increases phosphotriesterase activity of PON1 Homo sapiens
V346A the mutant shows a 4-16fold increase in PON1 activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
 delta-valerolactone recombinant enzyme Homo sapiens
0.6
-
 gamma-undecanoiclactone recombinant enzyme Homo sapiens
21
-
 gamma-butyrolactone recombinant enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ contains Ca2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the active site of the enzyme is characterized by two distinct binding regions, the hydrophobic binding site for arylesters/lactones and the paraoxon binding site for phosphotriesters ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver the enzyme is expressed mainly in the liver Homo sapiens
-
serum
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
delta-valerolactone + H2O
-
 Homo sapiens ?
-
 ?
gamma-butyrolactone + H2O
-
 Homo sapiens ?
-
 ?
gamma-undecanolactone + H2O
-
 Homo sapiens ?
-
 ?
additional information the active site of the enzyme is characterized by two distinct binding regions, the hydrophobic binding site for arylesters/lactones and the paraoxon binding site for phosphotriesters Homo sapiens ?
-
 ?
additional information the enzyme is most likely a lactonase, whereas the phosphotriesterase and esterase activities can be categorized as accidental Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
62
-
 gamma-undecanoiclactone recombinant enzyme Homo sapiens
111
-
 gamma-butyrolactone recombinant enzyme Homo sapiens
210
-
 delta-valerolactone recombinant enzyme Homo sapiens