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Literature summary for 3.1.1.25 extracted from

  • Billecke, S.; Draganov, D.; Counsell, R.; Stetson, P.; Watson, C.; Hsu, C.; La Du, B.N.
    Human serum paraoxonase (PON1) isozymes Q and R hydrolyze lactones and cyclic carbonate esters (2000), Drug Metab. Dispos., 28, 1335-1342.
    View publication on PubMed
Search for more literature for 3.1.1.25

Activating Compound

Activating Compound Comment Organism Structure
dilauroyl phosphatidylcholine stimulates activity of enzyme variants Q192 and R192 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C284A no lactonase activity with either homogentisic acid lactone or undecanoic-delta-lactone Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Mg2+ Homo sapiens
Zn2+ activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Zn2+ Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 dihydrocoumarin 25°C, enzyme variant R192 Homo sapiens
0.013
-
 2-coumaranone 25°C, enzyme variant R192 Homo sapiens
0.02
-
 2-coumaranone 25°C, enzyme variant Q192 Homo sapiens
0.022
-
 dihydrocoumarin 25°C, enzyme variant Q192 Homo sapiens
0.45
-
 Homogentisic acid lactone 25°C, enzyme variant R192 Homo sapiens
0.52
-
 Homogentisic acid lactone 25°C, enzyme variant Q192 Homo sapiens
2.8
-
 gamma-thiobutyrolactone 25°C, enzyme variant Q192 Homo sapiens
3.5
-
 gamma-thiobutyrolactone 25°C, enzyme variant R192 Homo sapiens
5.3
-
 gamma-butyrolactone 25°C, enzyme variant R192 Homo sapiens
15
-
 gamma-butyrolactone 25°C, enzyme variant Q192 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27169 variant Q192 and R192
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood serum
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,5S)-oxabicyclooctenone + H2O enzyme variant Q192: 0.82% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 0.75% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
(1S,5R)-oxabicyclooctenone + H2O enzyme variant Q192: 1.67% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 2.92% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
(R)-alpha-hydroxy-gamma-butyrolactone + H2O enzyme variant Q192: 0.88% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 3.95% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens (R)-2-hydroxybutyric acid
-
 ?
(R)-dihydro-5-(hydroxymethyl)-2(3H)-furanone + H2O enzyme variant Q192: 1.23% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 3.29% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
(S)-alpha-hydroxy-gamma-butyrolactone + H2O enzyme variant Q192: 8.14% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 19.6% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens (S)-2-hydroxybutyric acid
-
 ?
(S)-beta-hydroxy-gamma-butyrolactone + H2O enzyme variant Q192: 0.6% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 0.76% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens (S)-3-hydroxybutyric acid
-
 ?
(S)-dihydro-5-(hydroxymethyl)-2(3H)-furanone + H2O enzyme variant Q192: 0.78% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 1.12% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
2-coumaranone + H2O enzyme variant Q192: 18.3% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 13.5% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens (2-hydroxyphenyl)acetic acid
-
 ?
4-(1-propenyloxymethyl)-1,3-dioxolan-2-one + H2O enzyme variant Q192: 2.23% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 2.52% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
alpha-angelicalactone + H2O enzyme variant Q192: 19.9% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 14.8% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
alpha-bromo-gamma-butyrolactone + H2O enzyme variant Q192: 47.2% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 40.8% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 2-bromobutyric acid
-
 ?
beta-hydroxybutyrolactone + H2O enzyme variant Q192: 3.83% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 7.53% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
delta-decanolactone + H2O enzyme variant Q192: 9.65% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 12.8% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 5-hydroxydecanoic acid
-
 ?
delta-valerolactone + H2O enzyme variant Q192: 75.4% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 71.0% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 5-hydroxypentanoic acid
-
 ?
dihydrocoumarin + H2O enzyme variant Q192: 14.3% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 17% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 3-(2-hydroxyphenyl)propanoic acid
-
 ?
epsilon-caprolactone + H2O enzyme variant Q192: 14.8% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 25.7% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 6-hydroxyhexanoic acid
-
 ?
gamma-butyrolactone + H2O enzyme variant Q192: 2.46% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 9.05% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 4-hydroxybutyric acid
-
 ?
gamma-decanolactone + H2O enzyme variant Q192: 12.4% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 19.0% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 4-hydroxydecanoic acid
-
 ?
gamma-thiobutyrolactone + H2O
-
 Homo sapiens ?
-
 ?
gamma-valerolactone + H2O enzyme variant Q192: 7.28% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 6.97% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 4-hydroxypentanoic acid
-
 ?
homogentisic acid lactone + H2O enzyme variant Q192: 44% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 49.7% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens homogentisic acid
-
 ?
lovastatin + H2O activity with enzyme variant Q192 and R192 Homo sapiens ?
-
 ?
mevastatin + H2O activity with enzyme variant Q192 and R192 Homo sapiens ?
-
 ?
additional information enzyme protein also shows activities of paraoxonase (EC 3.1.8.1) and arylesterase (EC 3.1.1.2) Homo sapiens ?
-
 ?
propylene carbonate + H2O enzyme variant Q192: 5.02% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 8.8% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
simvastatin + H2O activity with enzyme variant Q192 and R192 Homo sapiens ?
-
 ?
spironolactone + H2O activity with enzyme variant Q192 and R192 Homo sapiens ?
-
 ?
undecano-gamma-lactone + H2O enzyme variant Q192: 11.8% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 12.7% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 4-hydroxyundecanoic acid
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens