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Literature summary for 3.1.1.23 extracted from

  • Tsurumura, T.; Tsuge, H.
    Substrate selectivity of bacterial monoacylglycerol lipase based on crystal structure (2014), J. Struct. Funct. Genomics, 15, 83-89.
    View publication on PubMed
Search for more literature for 3.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 1.5 M ammonium sulfate, 100 mM citrate (pH 5.8) and 100 mM tartrate Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.25
-
 arachidonoyl-1-thio-glycerol in 10 mM Tris-HCl (pH 7.2), temperature not specified in the publication Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) P82597
-
-
Bacillus sp. (in: Bacteria) H-257 P82597
-
-

Purification (Commentary)

Purification (Comment) Organism
octyl-Sepharose column chromatography, cation exchange column chromatography, and gel filtration Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-arachidonoylglycerol + H2O
-
 Bacillus sp. (in: Bacteria) glycerol + arachidonic acid
-
 ?
2-arachidonoylglycerol + H2O
-
 Bacillus sp. (in: Bacteria) H-257 glycerol + arachidonic acid
-
 ?
arachidonoyl-1-thio-glycerol + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
arachidonoyl-1-thio-glycerol + H2O
-
 Bacillus sp. (in: Bacteria) H-257 ?
-
 ?

Synonyms

Synonyms Comment Organism
MGL
-
 Bacillus sp. (in: Bacteria)
monoacylglycerol lipase
-
 Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.66
-
 arachidonoyl-1-thio-glycerol in 10 mM Tris-HCl (pH 7.2), temperature not specified in the publication Bacillus sp. (in: Bacteria)

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1
-
 arachidonoyl-1-thio-glycerol in 10 mM Tris-HCl (pH 7.2), temperature not specified in the publication Bacillus sp. (in: Bacteria)