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Literature summary for 3.1.1.23 extracted from

  • Rengachari, S.; Bezerra, G.A.; Riegler-Berket, L.; Gruber, C.C.; Sturm, C.; Taschler, U.; Boeszoermenyi, A.; Dreveny, I.; Zimmermann, R.; Gruber, K.; Oberer, M.
    The structure of monoacylglycerol lipase from Bacillus sp. H257 reveals unexpected conservation of the cap architecture between bacterial and human enzymes (2012), Biochim. Biophys. Acta, 1821, 1012-1021.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M MES/imidazole pH 6.5, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 12.5% (w/v) MPD Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
JZL184
-
Bacillus sp. (in: Bacteria)
phenylmethylsulfonyl fluoride
-
Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) P82597
-
-
Bacillus sp. (in: Bacteria) H-257 P82597
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Superdex 200 gel filtration Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
MGL
-
Bacillus sp. (in: Bacteria)
monoacylglycerol lipase
-
Bacillus sp. (in: Bacteria)