Application | Comment | Organism |
---|---|---|
synthesis | the highly stable Mortierella alliacea lipase may be useful for the synthesis of structured lipids, particularly acylglycerols containing functional unsaturated fatty acids at the sn-2 position | Mortierella alliacea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 50% residual activity at 1 mM | Mortierella alliacea | |
Co2+ | 47% residual activity at 1 mM | Mortierella alliacea | |
Cu2+ | 35% residual activity at 1 mM | Mortierella alliacea | |
diethyldicarbonate | 20% residual activity at 1 mM | Mortierella alliacea | |
diisopropylfluorophosphate | 75% residual activity at 1 mM | Mortierella alliacea | |
EDTA | 50% residual activity at 1 mM | Mortierella alliacea | |
Fe3+ | 35% residual activity at 1 mM | Mortierella alliacea | |
Hg2+ | 30% residual activity at 1 mM | Mortierella alliacea | |
Mn2+ | 58% residual activity at 1 mM | Mortierella alliacea | |
p-chloromercuribenzoic acid | 45% residual activity at 1 mM | Mortierella alliacea | |
phenylmethylsulfonyl fluoride | 70% residual activity at 1 mM | Mortierella alliacea | |
Zn2+ | 27% residual activity at 1 mM | Mortierella alliacea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 129% activity at 1 mM | Mortierella alliacea | |
additional information | not activated by K+ | Mortierella alliacea | |
Na+ | 110% activity at 1 mM | Mortierella alliacea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10965 | - |
x * 10965, MALDI-TOF mass spectrometry | Mortierella alliacea |
11000 | - |
x * 11000, SDS-PAGE | Mortierella alliacea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mortierella alliacea | - |
- |
- |
Mortierella alliacea YN-15 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
acetone precipitation, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration | Mortierella alliacea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29 | - |
crude enzyme, using olive oil as substrate, at pH 7.4, 30°C | Mortierella alliacea |
179 | - |
after 6.2fold purification, using olive oil as substrate, at pH 7.4, 30°C | Mortierella alliacea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-monocaprylin + H2O | - |
Mortierella alliacea | ? | - |
? | |
1-monolinolein + H2O | one of the most preferred substrates | Mortierella alliacea | ? | - |
? | |
1-monolinolenin + H2O | best substrate | Mortierella alliacea | ? | - |
? | |
1-monoolein + H2O | one of the most preferred substrates | Mortierella alliacea | ? | - |
? | |
1-monopalmitin + H2O | - |
Mortierella alliacea | ? | - |
? | |
2-monolinolein + H2O | - |
Mortierella alliacea | ? | - |
? | |
2-monoolein + H2O | - |
Mortierella alliacea | ? | - |
? | |
methylcaprylate + H2O | low activity | Mortierella alliacea | ? | - |
? | |
methyloleate + H2O | low activity | Mortierella alliacea | ? | - |
? | |
additional information | monoacylglycerols containing long-chain unsaturated fatty acids and phosphatidylcholine are preferentially hydrolyzed over triacylglycerols and fatty acid methyl esters. Triacylglycerol may be formed via 2-monoacylglycerol | Mortierella alliacea | ? | - |
? | |
additional information | the enzyme also uses soybean oil, sesame oil, rapeseed oil, camellia oil, olive oil and linseed oil as substrates. 1,3-dicaprylin, 1,3-dipalmitin, 1,2-diolein, 1,3-diolein, 1,2-dilinolein, methylbutyrate, methyllaurate, methylpalmitate, methylpamitoleate, methylsterate, methyllinoleate, methyllinolenate, and methyleicosapentaenoate yield no detectable activity at either 30 or 50°C | Mortierella alliacea | ? | - |
? | |
additional information | monoacylglycerols containing long-chain unsaturated fatty acids and phosphatidylcholine are preferentially hydrolyzed over triacylglycerols and fatty acid methyl esters. Triacylglycerol may be formed via 2-monoacylglycerol | Mortierella alliacea YN-15 | ? | - |
? | |
additional information | the enzyme also uses soybean oil, sesame oil, rapeseed oil, camellia oil, olive oil and linseed oil as substrates. 1,3-dicaprylin, 1,3-dipalmitin, 1,2-diolein, 1,3-diolein, 1,2-dilinolein, methylbutyrate, methyllaurate, methylpalmitate, methylpamitoleate, methylsterate, methyllinoleate, methyllinolenate, and methyleicosapentaenoate yield no detectable activity at either 30 or 50°C | Mortierella alliacea YN-15 | ? | - |
? | |
phosphatidylcholine + H2O | one of the most preferred substrates | Mortierella alliacea | ? | - |
? | |
phosphatidylcholine + H2O | one of the most preferred substrates | Mortierella alliacea YN-15 | ? | - |
? | |
tricaprylin + H2O | - |
Mortierella alliacea | ? | - |
? | |
tricaprylin + H2O | - |
Mortierella alliacea YN-15 | ? | - |
? | |
trieicosapentaenoin + H2O | low activity | Mortierella alliacea | ? | - |
? | |
trilaurin + H2O | low activity | Mortierella alliacea | ? | - |
? | |
trilinolein + H2O | low activity | Mortierella alliacea | ? | - |
? | |
trilinolenin + H2O | low activity | Mortierella alliacea | ? | - |
? | |
triolein + H2O | the lipase strongly hydrolyzes the sn-1/3 ester bonds and weakly hydrolyzes the sn-2 ester bonds of triolein | Mortierella alliacea | ? | - |
? | |
triolein + H2O | the lipase strongly hydrolyzes the sn-1/3 ester bonds and weakly hydrolyzes the sn-2 ester bonds of triolein | Mortierella alliacea YN-15 | ? | - |
? | |
tripalmitin + H2O | - |
Mortierella alliacea | ? | - |
? | |
tripalmitolein + H2O | - |
Mortierella alliacea | ? | - |
? | |
tristearin + H2O | - |
Mortierella alliacea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 11000, SDS-PAGE | Mortierella alliacea |
? | x * 10965, MALDI-TOF mass spectrometry | Mortierella alliacea |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Mortierella alliacea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Mortierella alliacea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Mortierella alliacea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
the enzyme is completely inactivated when preincubated for 1 h at over 70°C | Mortierella alliacea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Mortierella alliacea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 12 | - |
Mortierella alliacea |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1 | - |
in 50 mM phosphate buffer (pH 7.4), at 30°C | Mortierella alliacea | EDTA | |
1 | - |
in 50 mM phosphate buffer (pH 7.4), at 30°C | Mortierella alliacea | 2-mercaptoethanol |