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Literature summary for 3.1.1.23 extracted from

  • Jermsuntiea, W.; Aki, T.; Toyoura, R.; Iwashita, K.; Kawamoto, S.; Ono, K.
    Purification and characterization of intracellular lipase from the polyunsaturated fatty acid-producing fungus Mortierella alliacea (2011), New Biotechnol., 28, 158-164.
    View publication on PubMed

Application

Application Comment Organism
synthesis the highly stable Mortierella alliacea lipase may be useful for the synthesis of structured lipids, particularly acylglycerols containing functional unsaturated fatty acids at the sn-2 position Mortierella alliacea

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 50% residual activity at 1 mM Mortierella alliacea
Co2+ 47% residual activity at 1 mM Mortierella alliacea
Cu2+ 35% residual activity at 1 mM Mortierella alliacea
diethyldicarbonate 20% residual activity at 1 mM Mortierella alliacea
diisopropylfluorophosphate 75% residual activity at 1 mM Mortierella alliacea
EDTA 50% residual activity at 1 mM Mortierella alliacea
Fe3+ 35% residual activity at 1 mM Mortierella alliacea
Hg2+ 30% residual activity at 1 mM Mortierella alliacea
Mn2+ 58% residual activity at 1 mM Mortierella alliacea
p-chloromercuribenzoic acid 45% residual activity at 1 mM Mortierella alliacea
phenylmethylsulfonyl fluoride 70% residual activity at 1 mM Mortierella alliacea
Zn2+ 27% residual activity at 1 mM Mortierella alliacea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 129% activity at 1 mM Mortierella alliacea
additional information not activated by K+ Mortierella alliacea
Na+ 110% activity at 1 mM Mortierella alliacea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10965
-
x * 10965, MALDI-TOF mass spectrometry Mortierella alliacea
11000
-
x * 11000, SDS-PAGE Mortierella alliacea

Organism

Organism UniProt Comment Textmining
Mortierella alliacea
-
-
-
Mortierella alliacea YN-15
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration Mortierella alliacea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29
-
crude enzyme, using olive oil as substrate, at pH 7.4, 30°C Mortierella alliacea
179
-
after 6.2fold purification, using olive oil as substrate, at pH 7.4, 30°C Mortierella alliacea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-monocaprylin + H2O
-
Mortierella alliacea ?
-
?
1-monolinolein + H2O one of the most preferred substrates Mortierella alliacea ?
-
?
1-monolinolenin + H2O best substrate Mortierella alliacea ?
-
?
1-monoolein + H2O one of the most preferred substrates Mortierella alliacea ?
-
?
1-monopalmitin + H2O
-
Mortierella alliacea ?
-
?
2-monolinolein + H2O
-
Mortierella alliacea ?
-
?
2-monoolein + H2O
-
Mortierella alliacea ?
-
?
methylcaprylate + H2O low activity Mortierella alliacea ?
-
?
methyloleate + H2O low activity Mortierella alliacea ?
-
?
additional information monoacylglycerols containing long-chain unsaturated fatty acids and phosphatidylcholine are preferentially hydrolyzed over triacylglycerols and fatty acid methyl esters. Triacylglycerol may be formed via 2-monoacylglycerol Mortierella alliacea ?
-
?
additional information the enzyme also uses soybean oil, sesame oil, rapeseed oil, camellia oil, olive oil and linseed oil as substrates. 1,3-dicaprylin, 1,3-dipalmitin, 1,2-diolein, 1,3-diolein, 1,2-dilinolein, methylbutyrate, methyllaurate, methylpalmitate, methylpamitoleate, methylsterate, methyllinoleate, methyllinolenate, and methyleicosapentaenoate yield no detectable activity at either 30 or 50°C Mortierella alliacea ?
-
?
additional information monoacylglycerols containing long-chain unsaturated fatty acids and phosphatidylcholine are preferentially hydrolyzed over triacylglycerols and fatty acid methyl esters. Triacylglycerol may be formed via 2-monoacylglycerol Mortierella alliacea YN-15 ?
-
?
additional information the enzyme also uses soybean oil, sesame oil, rapeseed oil, camellia oil, olive oil and linseed oil as substrates. 1,3-dicaprylin, 1,3-dipalmitin, 1,2-diolein, 1,3-diolein, 1,2-dilinolein, methylbutyrate, methyllaurate, methylpalmitate, methylpamitoleate, methylsterate, methyllinoleate, methyllinolenate, and methyleicosapentaenoate yield no detectable activity at either 30 or 50°C Mortierella alliacea YN-15 ?
-
?
phosphatidylcholine + H2O one of the most preferred substrates Mortierella alliacea ?
-
?
phosphatidylcholine + H2O one of the most preferred substrates Mortierella alliacea YN-15 ?
-
?
tricaprylin + H2O
-
Mortierella alliacea ?
-
?
tricaprylin + H2O
-
Mortierella alliacea YN-15 ?
-
?
trieicosapentaenoin + H2O low activity Mortierella alliacea ?
-
?
trilaurin + H2O low activity Mortierella alliacea ?
-
?
trilinolein + H2O low activity Mortierella alliacea ?
-
?
trilinolenin + H2O low activity Mortierella alliacea ?
-
?
triolein + H2O the lipase strongly hydrolyzes the sn-1/3 ester bonds and weakly hydrolyzes the sn-2 ester bonds of triolein Mortierella alliacea ?
-
?
triolein + H2O the lipase strongly hydrolyzes the sn-1/3 ester bonds and weakly hydrolyzes the sn-2 ester bonds of triolein Mortierella alliacea YN-15 ?
-
?
tripalmitin + H2O
-
Mortierella alliacea ?
-
?
tripalmitolein + H2O
-
Mortierella alliacea ?
-
?
tristearin + H2O
-
Mortierella alliacea ?
-
?

Subunits

Subunits Comment Organism
? x * 11000, SDS-PAGE Mortierella alliacea
? x * 10965, MALDI-TOF mass spectrometry Mortierella alliacea

Synonyms

Synonyms Comment Organism
lipase
-
Mortierella alliacea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Mortierella alliacea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80
-
Mortierella alliacea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
the enzyme is completely inactivated when preincubated for 1 h at over 70°C Mortierella alliacea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Mortierella alliacea

pH Range

pH Minimum pH Maximum Comment Organism
2 12
-
Mortierella alliacea

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1
-
in 50 mM phosphate buffer (pH 7.4), at 30°C Mortierella alliacea EDTA
1
-
in 50 mM phosphate buffer (pH 7.4), at 30°C Mortierella alliacea 2-mercaptoethanol