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Literature summary for 3.1.1.23 extracted from
- A monoacylglycerol lipase from Mycobacterium smegmatis involved in bacterial cell interaction (2010), J. Bacteriol., 192, 4776-4785.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta(DE3)/pLysS cells | Mycolicibacterium smegmatis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S111A | inactive | Mycolicibacterium smegmatis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29882 | - |
x * 29882, calculated from amino acid sequence | Mycolicibacterium smegmatis |
| 30000 | - |
x * 30000, recombinant enzyme, SDS-PAGE | Mycolicibacterium smegmatis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
| Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 80 | - |
recombinant enzyme, using monomyristin as substrate, at 37°C and at pH 8.0 in 2.5 mM Tris-HCl buffer, 150 mM NaCl, and 3 mM sodium taurodeoxycholate | Mycolicibacterium smegmatis |
| 143 | - |
recombinant enzyme, using monomyristin as substrate, at 37°C and at pH 8.0 in 2.5 mM Tris-HCl buffer, 150 mM NaCl, and 3 mM sodium taurodeoxycholate | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| monomyristin + H2O | - |
Mycolicibacterium smegmatis | glycerol + myristate | - |
? |  |
| monomyristin + H2O | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | glycerol + myristate | - |
? | |
| monoolein + H2O | - |
Mycolicibacterium smegmatis | glycerol + oleate | - |
? | |
| monoolein + H2O | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | glycerol + oleate | - |
? | |
| additional information | no activity with triacetin, tributyrin, trioctanoin, triolein, dioctanoin, dimyristin, monooctanoin, diolein, and Tween 80 | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | no activity with triacetin, tributyrin, trioctanoin, triolein, dioctanoin, dimyristin, monooctanoin, diolein, and Tween 80 | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, recombinant enzyme, SDS-PAGE | Mycolicibacterium smegmatis |
| ? | x * 29882, calculated from amino acid sequence | Mycolicibacterium smegmatis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| monoacylglycerol lipase | - |
Mycolicibacterium smegmatis |
| MSMEG_0220 | - |
Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Mycolicibacterium smegmatis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | highly stable from pH 6.5 to 9.0 during 1 h of incubation. The enzyme shows a residual activity of 50% after 1 h of incubation at pH 4.0 | Mycolicibacterium smegmatis |
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Release 2023.1 (January 2023)
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