Cloned (Comment) | Organism |
---|---|
HeLa cells transiently transfected with pEF6 vector containing full-length ABHD6 or ABHD12 | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
celastrol | inhibits MGL activity, albeit less potently than pristimerin | Rattus norvegicus | |
dihydrocelastrol | inhibits MGL activity, albeit less potently than pristimerin | Rattus norvegicus | |
dihydrocelastryl diacetate | inhibits MGL activity, albeit less potently than pristimerin | Rattus norvegicus | |
euphol | inhibits MGL activity with high potency. Blocks MGL activity through a reversible and noncompetitive mechanism, which is apparently identical to that of pristimerin | Rattus norvegicus | |
pristimerin | inhibits MGL activity with high potency. Addition to purified MGL produces an almost immediate blockade of MGL activity. Inhibits MGL through a mechanism that is rapid, reversible, and noncompetitive | Rattus norvegicus | |
pristimerol | inhibits MGL activity, albeit less potently than pristimerin | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000014 | - |
2-oleoylglycerol | in the presence of 0.0005 mM pristimerin, at pH 7.5, 37°C | Rattus norvegicus | |
0.000024 | - |
2-oleoylglycerol | in the presence of 0.0003 mM pristimerin, at pH 7.5, 37°C | Rattus norvegicus | |
0.000027 | - |
2-oleoylglycerol | in the presence of 0.0001 mM pristimerin, at pH 7.5, 37°C | Rattus norvegicus | |
0.000028 | - |
2-oleoylglycerol | in the presence of vehicle (0.1% DMSO), at pH 7.5, 37°C | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar rats | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | MGL is localized to axon terminals throughout the brain | Rattus norvegicus | - |
embryo | day 18-20 | Rattus norvegicus | - |
neuron | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-arachidonoylglycerol + H2O | - |
Rattus norvegicus | arachidonic acid + glycerol | - |
? | |
2-oleoylglycerol + H2O | - |
Rattus norvegicus | oleic acid + glycerol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MGL | - |
Rattus norvegicus |
monoacylglycerol lipase | - |
Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000093 | - |
purified MGL, at pH 7.5, 37°C | Rattus norvegicus | pristimerin | |
0.000315 | - |
purified MG, at pH 7.5, 37°CL | Rattus norvegicus | euphol | |
0.000398 | - |
nonpurified MGL, at pH 7.5, 37°C | Rattus norvegicus | pristimerin | |
0.000882 | - |
nonpurified MGL, at pH 7.5, 37°C | Rattus norvegicus | euphol | |
0.0016 | - |
purified MGL, at pH 7.5, 37°C | Rattus norvegicus | celastrol | |
0.004 | - |
purified MGL, at pH 7.5, 37°C | Rattus norvegicus | pristimerol | |
0.015 | - |
purified MGL, at pH 7.5, 37°C | Rattus norvegicus | dihydrocelastrol | |
0.015 | - |
purified MGL, at pH 7.5, 37°C | Rattus norvegicus | dihydrocelastryl diacetate |