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Literature summary for 3.1.1.23 extracted from

  • Zvonok, N.; Williams, J.; Johnston, M.; Pandarinathan, L.; Janero, D.R.; Li, J.; Krishnan, S.C.; Makriyannis, A.
    Full mass spectrometric characterization of human monoacylglycerol lipase generated by large-scale expression and single-step purification (2008), J. Proteome Res., 7, 2158-2164.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0088
-
 arachidonoyl-7-hydroxy-6-methoxy-4-methylcoumarin ester recombinant enzyme, in TME buffer, at 37°C Homo sapiens
0.0197
-
 2-arachidonoylglycerol recombinant enzyme, in TME buffer, at 37°C Homo sapiens
0.0336
-
 2-arachidonoylglycerol native enzyme, in TME buffer, at 37°C Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-arachidonoylglycerol + H2O Rattus norvegicus
-
 arachidonic acid + glycerol
-
 ?
2-arachidonoylglycerol + H2O Homo sapiens MGL modulates endocannabinoid signaling in vivo by inactivating 2-arachidonoylglycerol (2-AG), the main endogenous agonist for central CB1 and peripheral CB2 cannabinoid receptors arachidonic acid + glycerol
-
 ?
arachidonoyl-7-hydroxy-6-methoxy-4-methylcoumarin ester + H2O Homo sapiens
-
 arachidonic acid + 7-hydroxy-6-methoxy-4-methylcoumarin
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q99685
-
-
Rattus norvegicus Q8R431
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-arachidonoylglycerol + H2O
-
 Rattus norvegicus arachidonic acid + glycerol
-
 ?
2-arachidonoylglycerol + H2O MGL modulates endocannabinoid signaling in vivo by inactivating 2-arachidonoylglycerol (2-AG), the main endogenous agonist for central CB1 and peripheral CB2 cannabinoid receptors Homo sapiens arachidonic acid + glycerol
-
 ?
arachidonoyl-7-hydroxy-6-methoxy-4-methylcoumarin ester + H2O
-
 Homo sapiens arachidonic acid + 7-hydroxy-6-methoxy-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
MGL
-
 Rattus norvegicus
MGL
-
 Homo sapiens
monoacylglycerol lipase
-
 Rattus norvegicus
monoacylglycerol lipase
-
 Homo sapiens