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Literature summary for 3.1.1.23 extracted from

  • Sakiyama, T.; Yoshimi, T.; Miyake, A.; Umeoka, M.; Tanaka, A.; Ozaki, S.; Nakanishi, K.
    Purification and characterization of a monoacylglycerol lipase from Pseudomonas sp. LP7315 (2001), J. Biosci. Bioeng., 91, 27-32.
    View publication on PubMed
Search for more literature for 3.1.1.23

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. LP7315
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
109
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-monolinoleoylglycerol + H2O
-
 Pseudomonas sp. linolic acid + glycerol
-
 ?
1-monomyristoylglycerol + H2O best substrate Pseudomonas sp. myristic acid + glycerol
-
 r
1-monomyristoylglycerol + H2O best substrate Pseudomonas sp. LP7315 myristic acid + glycerol
-
 r
1-monooleoylglycerol + H2O
-
 Pseudomonas sp. oleic acid + glycerol
-
 ?
1-monooleoylglycerol + H2O
-
 Pseudomonas sp. LP7315 oleic acid + glycerol
-
 ?
1-monopalmitoylglycerol + H2O
-
 Pseudomonas sp. palmitic acid + glycerol
-
 r
1-monopalmitoylglycerol + H2O
-
 Pseudomonas sp. LP7315 palmitic acid + glycerol
-
 r
4-nitrophenyllaurate + H2O
-
 Pseudomonas sp. 4-nitrophenol + lauric acid
-
 r
4-nitrophenyllaurate + H2O
-
 Pseudomonas sp. LP7315 4-nitrophenol + lauric acid
-
 r
additional information enzyme also catalyzes monoglyceride synthesis in a solvent-free two-phase system, in which fatty acid droplets are dispersed in the glycerol phase with a low water content, enzyme does not hydrolyze di- and triglycerides Pseudomonas sp. ?
-
 ?
additional information enzyme also catalyzes monoglyceride synthesis in a solvent-free two-phase system, in which fatty acid droplets are dispersed in the glycerol phase with a low water content, enzyme does not hydrolyze di- and triglycerides Pseudomonas sp. LP7315 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 stable for 1h Pseudomonas sp.
70
-
 80% activity after 1h Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas sp.