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Literature summary for 3.1.1.23 extracted from

  • Imamura, S.; Kitaura, S.
    Purification and characterization of a monoacylglycerol lipase from the moderately thermophilic Bacillus sp. H-257 (2000), J. Biochem., 127, 419-425.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
deoxycholate 17% inhibition at 5 mM Bacillus sp. (in: Bacteria)
Triton X-100 10% inhibition at 0.25% Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.059
-
2-monooleoylglycerol pH 7.3, 37°C Bacillus sp. (in: Bacteria)
0.083
-
1-monooleoylglycerol pH 7.3, 37°C Bacillus sp. (in: Bacteria)
0.14
-
1-monolauroylglycerol pH 7.3, 37°C Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) H-257
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
121.1
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-monolauroylglycerol + H2O best substrate Bacillus sp. (in: Bacteria) lauric acid + glycerol
-
?
1-monolauroylglycerol + H2O best substrate Bacillus sp. (in: Bacteria) H-257 lauric acid + glycerol
-
?
1-monooleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) oleic acid + glycerol
-
?
1-monooleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) H-257 oleic acid + glycerol
-
?
2-monooleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) oleic acid + glycerol
-
?
2-monooleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) H-257 oleic acid + glycerol
-
?
monolinoleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) linoic acid + glycerol
-
?
monolinoleoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) H-257 linoic acid + glycerol
-
?
monomyristoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) myristic acid + glycerol
-
?
monostearoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) stearic acid + glycerol
-
?
monostearoylglycerol + H2O
-
Bacillus sp. (in: Bacteria) H-257 stearic acid + glycerol
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable for 10 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria) isoelectric focusing, pH gradient 3.5-10 4.8 4.7