Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
A76G | reduced thermostability, increased stability at pH 5-7, substitution converts the esterase into a monoacylglycerol hydrolase | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19490 | - |
calculated from amino acid sequence | Bacillus subtilis |
20500 | - |
SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q79F14 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
200 | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl caprate + H2O | - |
Bacillus subtilis | 4-nitrophenol + capric acid | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Bacillus subtilis | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl-caproate + H2O | - |
Bacillus subtilis | 4-nitrophenol + caproic acid | - |
? | |
4-nitrophenyl-laurate + H2O | - |
Bacillus subtilis | 4-nitrophenol + lauric acid | - |
? | |
4-nitrophenyl-myristate + H2O | - |
Bacillus subtilis | 4-nitrophenol + myristic acid | - |
? | |
4-nitrophenyl-palmitate + H2O | - |
Bacillus subtilis | 4-nitrophenol + palmitic acid | - |
? | |
4-nitrophenyl-stearate + H2O | - |
Bacillus subtilis | 4-nitrophenol + stearic acid | - |
? | |
additional information | preferentially hydrolyzes triacylglycerol-esters and 4-nitrophenyl-esters of fatty acids with short chain lengths up to 10 carbon atoms, no hydrolysis of triolein | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LipB | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
wild-type enzyme is stable, A76G mutant loses 50% activity after 8 min | Bacillus subtilis |