Literature summary for 3.1.1.22 extracted from

Arikawa, H.; Sato, S.; Fujiki, T.; Matsumoto, K.
A study on the relation between poly(3-hydroxybutyrate) depolymerases or oligomer hydrolases and molecular weight of polyhydroxyalkanoates accumulating in Cupriavidus necator H16 (2016), J. Biotechnol., 227, 94-102 .

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Protein Variants

Protein Variants	Comment	Organism
additional information	recombinant Cupriavidus necator strain KNK-005 harbors an NSDG mutant of the PHA synthase gene (phaCAc) from Aeromonas caviae. Deletion of phaZ1 (EC 3.1.1.75, UniProt ID Q0KCI0) and phaZ2 has a significant and slight attenuating effect, respectively, on the reduction in polyhydroxyalkanoate (PHA) content of KNK-005 cells. Regardless of the PHA consumption, its molecular weight does not decrease. The DELTAphaZ1DELTAphaZ2DELTAphaZ6 triple mutant of KNK-005, 005dZ126, is a promising strain capable of producing ultra-high-molecular-weight poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) of ultra-high-molecular-weight and barely degrades PHBHHx and polyhydroxyalkanoate enzymatically intracellularly	Cupriavidus necator

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Cupriavidus necator	5622	-

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator	Q0K7T2	i.e. Alcaligenes eutrophus or Ralstonia eutropha	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme shows no activity with 3HB oligomers or poly(hydroxybutyrate), PHB, granules	Cupriavidus necator	?	-	?

Synonyms

Synonyms	Comment	Organism
D-(-)-3-hydroxybutyrate oligomer hydrolase	UniProt	Cupriavidus necator
H16 A2862	locus name	Cupriavidus necator
PhaZ2	-	Cupriavidus necator

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Release 2023.1 (January 2023)