Literature summary for 3.1.1.22 extracted from

York, G.M.; Lupberger, J.; Tian, J.; Lawrence, A.G.; Stubbe, J.; Sinskey, A.J.
Ralstonia eutropha H16 encodes two and possibly three intracellular poly[D-(-)-3-hydroxybutyrate] depolymerase genes (2003), J. Bacteriol., 185, 3788-3794 .

Search for more literature for 3.1.1.22

Cloned(Commentary)

Cloned (Comment)	Organism
gene phaZ1, pha sequences comparisons, subcloning in Escherichia coli strains DH5alpha and S17-1	Cupriavidus necator
gene phaZ2, pha sequences comparisons, subcloning in strains DH5alpha and S17-1	Cupriavidus necator

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of a set of phaZ gene deletion strains, i.e. DELTAphaZ1, DELTAphaZ2, DELTAphaZ3, DELTAphaZ1DELTAphaZ2, DELTAphaZ1DELTAphaZ3, DELTAphaZ2DELTAphaZ3, and DELTAphaZ1DELTAphaZ2DELTAphaZ3 from strain H16. All mutant strains are unable to produce poly(hydroxybutyrate)	Cupriavidus necator

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Cupriavidus necator	5622	-
additional information	the enzyme sequence contains no signal sequence	Cupriavidus necator	-	-

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator	O87189	i.e. Alcaligenes eutrophus or Ralstonia eutropha	-
Cupriavidus necator	Q7WT49	i.e. Alcaligenes eutrophus or Ralstonia eutropha	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme PhaZ1 converts the polymer into oligomers and monomers of hydroxybutyrate by the end of the degradation process	Cupriavidus necator	?	-	?

Synonyms

Synonyms	Comment	Organism
intracellular PHB depolymerase	UniProt	Cupriavidus necator
More	cf. EC 3.1.1.75	Cupriavidus necator
PhaZ	-	Cupriavidus necator
PhaZ1	-	Cupriavidus necator
PhaZ2	-	Cupriavidus necator
phaZre	-	Cupriavidus necator
poly[D-(-)-3-hydroxybutyrate] depolymerase	-	Cupriavidus necator

General Information

General Information	Comment	Organism
additional information	enzyme PhaZ1 has neither a lipase box nor an identifiable PHB binding domain	Cupriavidus necator

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Release 2023.1 (January 2023)