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Literature summary for 3.1.1.22 extracted from

  • Uchino, K.; Katsumata, Y.; Takeda, T.; Arai, H.; Shiraki, M.; Saito, T.
    Purification and molecular cloning of an intracellular 3-hydroxybutyrate-oligomer hydrolase from Paucimonas lemoignei (2007), J. Biosci. Bioeng., 104, 224-226.
    View publication on PubMed
Search for more literature for 3.1.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paucimonas lemoignei

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by 1,4-dimercapto-2,3-butanediol; not inhibited by (p-tert-octylphenoxy)polyethoxyethanol Paucimonas lemoignei
Phenylmethylsulfonylfluoride 10 mM, 50% enzyme activity inhibited Paucimonas lemoignei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate
-
 Paucimonas lemoignei
0.85
-
 (R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
-
 Paucimonas lemoignei
1.1
-
 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate
-
 Paucimonas lemoignei

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 estimated by SDS-PAGE, deduced from sequence of cDNA Paucimonas lemoignei

Organism

Organism UniProt Comment Textmining
Paucimonas lemoignei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hydrophobic column chromatography Paucimonas lemoignei

Storage Stability

Storage Stability Organism
-20°C, NaCl 0.4 M, 6 months Paucimonas lemoignei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
-
 Paucimonas lemoignei (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O
-
 Paucimonas lemoignei (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
-
 Paucimonas lemoignei (R)-3-hydroxybutanoate
-
 ?

Synonyms

Synonyms Comment Organism
3-hydroxybutyrate-oligomer hydrolase
-
 Paucimonas lemoignei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
10
-
-
 Paucimonas lemoignei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22
-
 (R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
-
 Paucimonas lemoignei
80
-
 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate
-
 Paucimonas lemoignei
1500
-
 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate
-
 Paucimonas lemoignei