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Literature summary for 3.1.1.22 extracted from

  • Sugiyama, A.; Kobayashi, T.; Shiraki, M.; Saito, T.
    Roles of poly(3-hydroxybutyrate) depolymerase and 3HB-oligomer hydrolase in bacterial PHB metabolism (2004), Curr. Microbiol., 48, 424-427.
    View publication on PubMed
Search for more literature for 3.1.1.22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 (R)-3-((R)-3-hydroxybutanoloxy)butanoate
-
 Cupriavidus necator
0.61
-
 (R)-3-((R)-3-hydroxybutanoloxy)butanoate
-
 Ralstonia pickettii
0.7
-
 (R)-3-((R)-3-hydroxybutanoloxy)butanoate
-
 Acidovorax sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Ralstonia pickettii
-
-
intracellular
-
 Cupriavidus necator 5622
-
intracellular
-
 Acidovorax sp. 5622
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O Cupriavidus necator involved in the degradation of poly-3-hydroxybutyrate (PHB) (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O Ralstonia pickettii involved in the degradation of poly-3-hydroxybutyrate (PHB) (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O Acidovorax sp. involved in the degradation of poly-3-hydroxybutyrate (PHB) (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 involved in the degradation of poly-3-hydroxybutyrate (PHB) (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O Ralstonia pickettii T1 involved in the degradation of poly-3-hydroxybutyrate (PHB) (R)-3-hydroxybutanoate
-
 ?
additional information Cupriavidus necator hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency ?
-
 ?
additional information Ralstonia pickettii hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency ?
-
 ?
additional information Acidovorax sp. hydrolyzes exclusively 3-hydroxybutyrate-dimers and trimers, no hydrolysis of longer linear or cyclic oligomers ?
-
 ?
additional information Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency ?
-
 ?
additional information Ralstonia pickettii T1 hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency ?
-
 ?

Organism

Organism UniProt Comment Textmining
Acidovorax sp.
-
-
-
Cupriavidus necator
-
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
Ralstonia pickettii
-
-
-
Ralstonia pickettii T1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
-
 Cupriavidus necator (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
-
 Ralstonia pickettii (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
-
 Acidovorax sp. (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O involved in the degradation of poly-3-hydroxybutyrate (PHB) Cupriavidus necator (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O involved in the degradation of poly-3-hydroxybutyrate (PHB) Ralstonia pickettii (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O involved in the degradation of poly-3-hydroxybutyrate (PHB) Acidovorax sp. (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O involved in the degradation of poly-3-hydroxybutyrate (PHB) Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
-
 Ralstonia pickettii T1 (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O involved in the degradation of poly-3-hydroxybutyrate (PHB) Ralstonia pickettii T1 (R)-3-hydroxybutanoate
-
 ?
additional information hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency Cupriavidus necator ?
-
 ?
additional information hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency Ralstonia pickettii ?
-
 ?
additional information hydrolyzes exclusively 3-hydroxybutyrate-dimers and trimers, no hydrolysis of longer linear or cyclic oligomers Acidovorax sp. ?
-
 ?
additional information hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
additional information hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency Ralstonia pickettii T1 ?
-
 ?

Synonyms

Synonyms Comment Organism
3-HB-oligomer hydrolase
-
 Cupriavidus necator
3-HB-oligomer hydrolase
-
 Ralstonia pickettii
3-HB-oligomer hydrolase
-
 Acidovorax sp.
3HBOH
-
 Cupriavidus necator
3HBOH
-
 Ralstonia pickettii
3HBOH
-
 Acidovorax sp.