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Literature summary for 3.1.1.20 extracted from

  • Hatamoto, O.; Watarai, T.; Kikuchi, M.; Mizusawa, K.; Sekine, H.
    Cloning and sequencing of the gene encoding tannase and a structural study of the tannase subunit from Aspergillus oryzae (1996), Gene, 175, 215-221.
    View publication on PubMed
Search for more literature for 3.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Escherichia coli JM 109 and expressed in Aspergillus oryzae strain AO1, a tannase low-producing and nitrate reductase-deficient strain Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000 + x * 33000, four pairs of two subunits form a hetero-oligomer of a about 300000 Da native tannase, SDS-PAGE Aspergillus oryzae
33000
-
 x * 30000 + x * 33000, four pairs of two subunits form a hetero-oligomer of a about 300000 Da native tannase, SDS-PAGE Aspergillus oryzae
290000
-
 strain AO1, glycoprotein containing 22.7% sugars, a hetero-octamer with four pairs of two subunits, sedimentation equilibrium Aspergillus oryzae
310000
-
 strain AO1, glycoprotein containing 22.7% sugars, a hetero-octamer with four pairs of two subunits, gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Aspergillus oryzae
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified from the strain AO1 Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tannic acid + H2O
-
 Aspergillus oryzae gallic acid + ?
-
 ?
tannic acid + H2O
-
 Aspergillus oryzae ?
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 30000 + x * 33000, four pairs of two subunits form a hetero-oligomer of a about 300000 Da native tannase, SDS-PAGE Aspergillus oryzae