Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropylfluorophosphate | 83% inhibition | Aspergillus niger | |
additional information | not inhibited by o-phenanthroline, PMSF, EDTA, 2-mercaptomethanol, sodium thioglycolate | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | CaCl2, no effect on the activity | Aspergillus niger | |
Co2+ | CoCl2 no effect on activity | Aspergillus niger | |
Cu2+ | CuSO4: 68% inhibition | Aspergillus niger | |
FeCl3 | 13% inhibition | Aspergillus niger | |
Mg2+ | MgSO4 no effect on activity | Aspergillus niger | |
Mn2+ | MnCl2 no effect on activity | Aspergillus niger | |
Zn2+ | ZnCl2 39% inhibition | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Aspergillus niger |
186000 | - |
glycoprotein containing 43% sugars, nondenaturing PAGE | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | Aspergillus niger | - |
? | - |
? | |
tannic acid + H2O | Aspergillus niger LCF 8 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
LCF 8 | - |
Aspergillus niger LCF 8 | - |
LCF 8 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | glycoprotein, containg 43% sugars | Aspergillus niger |
Purification (Comment) | Organism |
---|---|
29.1fold | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
118.8 | - |
3 step purification | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | - |
Aspergillus niger | gallic acid + ? | - |
? | |
tannic acid + H2O | - |
Aspergillus niger LCF 8 | gallic acid + ? | - |
? | |
tannic acid + H2O | - |
Aspergillus niger | ? | - |
? | |
tannic acid + H2O | - |
Aspergillus niger LCF 8 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | active between these temperatures | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 45 | stable between | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
pI 4.3 | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | - |
Aspergillus niger |