Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 25% inhibition at 10 mM | Aspergillus oryzae | |
Cu2+ | 53% inhibition at 10 mM | Aspergillus oryzae | |
Fe2+ | 55% inhibition at 10 mM | Aspergillus oryzae | |
Hg2+ | 73% inhibition at 10 mM | Aspergillus oryzae | |
Zn2+ | 11% inhibition at 10 mM | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics analysis, temperature dependence of kinetic parameters on tannic acid hydrolysis, overview | Aspergillus oryzae | |
4.13 | - |
Tannic acid | pH 5.0, 37°C | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Aspergillus oryzae | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates 32% at 10 mM | Aspergillus oryzae | |
additional information | poorly activating effect by Mg2+ at 10 mM | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106000 | - |
gel filtration and native PAGE | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | Aspergillus oryzae | - |
gallic acid + D-glucose | - |
? | |
tannic acid + H2O | Aspergillus oryzae RIB 40 | - |
gallic acid + D-glucose | - |
? | |
tannic acid + H2O | Aspergillus oryzae ATCC 42149 | - |
gallic acid + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | P78581 | from China green tea paste | - |
Aspergillus oryzae ATCC 42149 | P78581 | from China green tea paste | - |
Aspergillus oryzae RIB 40 | P78581 | from China green tea paste | - |
Purification (Comment) | Organism |
---|---|
native intracellular enzyme 36.7fold from submerged culture by ammonium sulfate fractionation, dialysis and desalting gel filtration, and two steps of gel filtration | Aspergillus oryzae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | submerged growth conditions | Aspergillus oryzae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2453.9 | - |
purified enzyme, pH 5.0, 37°C, substrate tannic acid | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | tannase hydrolyzes different tannin-rich substrates. The highest relative activity is recorded for tannic acid (100%) followed by extract of china green tea (83.3%). Lower activity is obtained for the other tannin extracts of commercial red tea (42.3%), bark of Acacia nilotica (37.4%), leaves of Acacia nilotica (29.8%), bark of Acacia saligna (13.5%) and bark of Acacia ehrenbergina (4.2%). The high relative activity recorded for China green tea extract may be attributed to its richness with gallotannins mainly epigallocatechin gallate | Aspergillus oryzae | ? | - |
? | |
additional information | tannase hydrolyzes different tannin-rich substrates. The highest relative activity is recorded for tannic acid (100%) followed by extract of china green tea (83.3%). Lower activity is obtained for the other tannin extracts of commercial red tea (42.3%), bark of Acacia nilotica (37.4%), leaves of Acacia nilotica (29.8%), bark of Acacia saligna (13.5%) and bark of Acacia ehrenbergina (4.2%). The high relative activity recorded for China green tea extract may be attributed to its richness with gallotannins mainly epigallocatechin gallate | Aspergillus oryzae RIB 40 | ? | - |
? | |
additional information | tannase hydrolyzes different tannin-rich substrates. The highest relative activity is recorded for tannic acid (100%) followed by extract of china green tea (83.3%). Lower activity is obtained for the other tannin extracts of commercial red tea (42.3%), bark of Acacia nilotica (37.4%), leaves of Acacia nilotica (29.8%), bark of Acacia saligna (13.5%) and bark of Acacia ehrenbergina (4.2%). The high relative activity recorded for China green tea extract may be attributed to its richness with gallotannins mainly epigallocatechin gallate | Aspergillus oryzae ATCC 42149 | ? | - |
? | |
tannic acid + H2O | - |
Aspergillus oryzae | gallic acid + D-glucose | - |
? | |
tannic acid + H2O | - |
Aspergillus oryzae RIB 40 | gallic acid + D-glucose | - |
? | |
tannic acid + H2O | - |
Aspergillus oryzae ATCC 42149 | gallic acid + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | x * 106000, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
TanA | - |
Aspergillus oryzae |
tannin acyl hydrolase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature dependence of kinetic parameters on tannic acid hydrolysis, overview | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | purified native enzyme, the calculated half-life times at 40, 45, 50, 55, 60, and 70°C are 955.15, 142.0, 30.28, 17.88, 8.23, and 2.95 min, respectively, determination of thermodynamic parameters for irreversible thermal inactivation at 40-70°C | Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
551.4 | - |
Tannic acid | pH 5.0, 37°C | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | activity range, profile overview | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8.5 | purified native enzyme, 25°C, 180 min, over 50% activity remaining, completely stable at pH 4.5-7.0 | Aspergillus oryzae |
General Information | Comment | Organism |
---|---|---|
physiological function | tannase catalyses the breakdown of ester and depside link-ages in hydrolysable tannins such as tannic acid, producing gallic acid and glucose | Aspergillus oryzae |