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Literature summary for 3.1.1.20 extracted from

  • Abdel-Naby, M.A.; El-Tanash, A.B.; Sherief, A.D.
    Structural characterization, catalytic, kinetic and thermodynamic properties of Aspergillus oryzae tannase (2016), Int. J. Biol. Macromol., 92, 803-811 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 25% inhibition at 10 mM Aspergillus oryzae
Cu2+ 53% inhibition at 10 mM Aspergillus oryzae
Fe2+ 55% inhibition at 10 mM Aspergillus oryzae
Hg2+ 73% inhibition at 10 mM Aspergillus oryzae
Zn2+ 11% inhibition at 10 mM Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics and thermodynamics analysis, temperature dependence of kinetic parameters on tannic acid hydrolysis, overview Aspergillus oryzae
4.13
-
Tannic acid pH 5.0, 37°C Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Aspergillus oryzae 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 32% at 10 mM Aspergillus oryzae
additional information poorly activating effect by Mg2+ at 10 mM Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
gel filtration and native PAGE Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Aspergillus oryzae
-
gallic acid + D-glucose
-
?
tannic acid + H2O Aspergillus oryzae RIB 40
-
gallic acid + D-glucose
-
?
tannic acid + H2O Aspergillus oryzae ATCC 42149
-
gallic acid + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae P78581 from China green tea paste
-
Aspergillus oryzae ATCC 42149 P78581 from China green tea paste
-
Aspergillus oryzae RIB 40 P78581 from China green tea paste
-

Purification (Commentary)

Purification (Comment) Organism
native intracellular enzyme 36.7fold from submerged culture by ammonium sulfate fractionation, dialysis and desalting gel filtration, and two steps of gel filtration Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
mycelium submerged growth conditions Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2453.9
-
purified enzyme, pH 5.0, 37°C, substrate tannic acid Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information tannase hydrolyzes different tannin-rich substrates. The highest relative activity is recorded for tannic acid (100%) followed by extract of china green tea (83.3%). Lower activity is obtained for the other tannin extracts of commercial red tea (42.3%), bark of Acacia nilotica (37.4%), leaves of Acacia nilotica (29.8%), bark of Acacia saligna (13.5%) and bark of Acacia ehrenbergina (4.2%). The high relative activity recorded for China green tea extract may be attributed to its richness with gallotannins mainly epigallocatechin gallate Aspergillus oryzae ?
-
?
additional information tannase hydrolyzes different tannin-rich substrates. The highest relative activity is recorded for tannic acid (100%) followed by extract of china green tea (83.3%). Lower activity is obtained for the other tannin extracts of commercial red tea (42.3%), bark of Acacia nilotica (37.4%), leaves of Acacia nilotica (29.8%), bark of Acacia saligna (13.5%) and bark of Acacia ehrenbergina (4.2%). The high relative activity recorded for China green tea extract may be attributed to its richness with gallotannins mainly epigallocatechin gallate Aspergillus oryzae RIB 40 ?
-
?
additional information tannase hydrolyzes different tannin-rich substrates. The highest relative activity is recorded for tannic acid (100%) followed by extract of china green tea (83.3%). Lower activity is obtained for the other tannin extracts of commercial red tea (42.3%), bark of Acacia nilotica (37.4%), leaves of Acacia nilotica (29.8%), bark of Acacia saligna (13.5%) and bark of Acacia ehrenbergina (4.2%). The high relative activity recorded for China green tea extract may be attributed to its richness with gallotannins mainly epigallocatechin gallate Aspergillus oryzae ATCC 42149 ?
-
?
tannic acid + H2O
-
Aspergillus oryzae gallic acid + D-glucose
-
?
tannic acid + H2O
-
Aspergillus oryzae RIB 40 gallic acid + D-glucose
-
?
tannic acid + H2O
-
Aspergillus oryzae ATCC 42149 gallic acid + D-glucose
-
?

Subunits

Subunits Comment Organism
monomer x * 106000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
TanA
-
Aspergillus oryzae
tannin acyl hydrolase
-
Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
temperature dependence of kinetic parameters on tannic acid hydrolysis, overview Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 70 purified native enzyme, the calculated half-life times at 40, 45, 50, 55, 60, and 70°C are 955.15, 142.0, 30.28, 17.88, 8.23, and 2.95 min, respectively, determination of thermodynamic parameters for irreversible thermal inactivation at 40-70°C Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
551.4
-
Tannic acid pH 5.0, 37°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
3 8 activity range, profile overview Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 8.5 purified native enzyme, 25°C, 180 min, over 50% activity remaining, completely stable at pH 4.5-7.0 Aspergillus oryzae

General Information

General Information Comment Organism
physiological function tannase catalyses the breakdown of ester and depside link-ages in hydrolysable tannins such as tannic acid, producing gallic acid and glucose Aspergillus oryzae