Protein Variants | Comment | Organism |
---|---|---|
additional information | preparation of tannase immobilized onto carboxyl-functionalized superparamagnetic ferroferric oxide nanoparticles (CMNPs) by glutaraldehyde (best at 3.5% v/v), method evaluation and optimization, overview. The activity of the immobilized tannase reaches 85.35 U/g carrier after 24 h of immobilization. Successful binding between CMNPs and tannase is confirmed by Fourier transform infrared spectroscopy and thermogravimetric analysis. Vibrating sample magnetometry and X-ray diffraction show that the CMNPs and immobilized tannase exhibit distinct magnetic responses and superparamagnetic properties. The thermal, pH, and storage stabilities of the immobilized tannase are superior to those of free tannase. Immobilized tannase is more stable than free tannase at pH -8 with over 80% residual enzyme activity. After six cycles of catalytic hydrolysis of propyl gallate, the immobilized tannase maintains over 60% of its initial activity. The Michaelis constant (Km) of the immobilized enzyme indicates its higher affinity for substrate binding than the free enzyme | Aspergillus tubingensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Aspergillus tubingensis | |
0.29 | - |
propyl gallate | immobilized enzyme, pH 5.0, 30°C | Aspergillus tubingensis | |
0.45 | - |
propyl gallate | free enzyme, pH 5.0, 30°C | Aspergillus tubingensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus tubingensis | - |
- |
- |
Aspergillus tubingensis CICC 2651 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | for activity detection, the produced gallic acid is combined with alcoholic rhodanine to form a chromogen, that is spectrometrically measured | Aspergillus tubingensis | ? | - |
? | |
additional information | for activity detection, the produced gallic acid is combined with alcoholic rhodanine to form a chromogen, that is spectrometrically measured | Aspergillus tubingensis CICC 2651 | ? | - |
? | |
propyl gallate + H2O | - |
Aspergillus tubingensis | propanol + gallic acid | - |
? | |
propyl gallate + H2O | - |
Aspergillus tubingensis CICC 2651 | propanol + gallic acid | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
free and immobilized enzyme | Aspergillus tubingensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | activity range | Aspergillus tubingensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
free enzyme | Aspergillus tubingensis |
7 | - |
immobilized enzyme | Aspergillus tubingensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | activity range | Aspergillus tubingensis |