Cloned (Comment) | Organism |
---|---|
gene tanA, recombinant expression of wild-type and mutant enzymes in Pichia pastoris strain GS115, recombinant AoTanA and its mutant variants are secreted using a Saccharomyces cerevisiae alpha-factor secretion signal peptide fusion, the recombinant proteins accumulate in the culture broth | Aspergillus oryzae |
Protein Variants | Comment | Organism |
---|---|---|
C194A | site-directed mutagenesis, almost inactive mutant | Aspergillus oryzae |
C502A | site-directed mutagenesis, almost inactive mutant | Aspergillus oryzae |
additional information | construction of a seven amino-acid deletion variant of region Lys310eArg316 (mutant DELTAKR). The mutant shows only one band by SDS-PAGE after treatment with endoglycosidase H. The DELTAKR variant exhibits higher activity compared to the wild-type enzyme | Aspergillus oryzae |
R311A/R316A | site-directed mutagenesis, the mutant exhibits the two bands similar to wild-type by SDS-PAGE after treatment with endoglycosidase H. The mutation has no effect on tannase activity and stability | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | P78581 | - |
- |
Aspergillus oryzae ATCC 42149 | P78581 | - |
- |
Aspergillus oryzae RIB 40 | P78581 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme consists of two subunits that are generated by the cleavage of tannase gene product by the Kex2 protease, TanA has two Kex2 recognition sites at positions Arg311 and Arg316 | Aspergillus oryzae |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Pichia pastoris strain GS115 by anion exchange chromatography, ultrafiltration, and gel filtration | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-catechin gallate + H2O | high activity | Aspergillus oryzae | (-)-catechin + gallic acid | - |
? | |
(-)-catechin gallate + H2O | high activity | Aspergillus oryzae RIB 40 | (-)-catechin + gallic acid | - |
? | |
(-)-catechin gallate + H2O | high activity | Aspergillus oryzae ATCC 42149 | (-)-catechin + gallic acid | - |
? | |
(-)-epicatechin gallate + H2O | best substrate | Aspergillus oryzae | (-)-epicatechin + gallic acid | - |
? | |
(-)-epicatechin gallate + H2O | best substrate | Aspergillus oryzae RIB 40 | (-)-epicatechin + gallic acid | - |
? | |
(-)-epicatechin gallate + H2O | best substrate | Aspergillus oryzae ATCC 42149 | (-)-epicatechin + gallic acid | - |
? | |
(-)-epigallocatechin gallate + H2O | moderate activity | Aspergillus oryzae | (-)-epigallocatechin + gallic acid | - |
? | |
(-)-epigallocatechin gallate + H2O | moderate activity | Aspergillus oryzae RIB 40 | (-)-epigallocatechin + gallic acid | - |
? | |
(-)-epigallocatechin gallate + H2O | moderate activity | Aspergillus oryzae ATCC 42149 | (-)-epigallocatechin + gallic acid | - |
? | |
ethyl gallate + H2O | lower activity | Aspergillus oryzae | ethanol + gallic acid | - |
? | |
ethyl gallate + H2O | lower activity | Aspergillus oryzae RIB 40 | ethanol + gallic acid | - |
? | |
ethyl gallate + H2O | lower activity | Aspergillus oryzae ATCC 42149 | ethanol + gallic acid | - |
? | |
methyl gallate + H2O | moderate activity | Aspergillus oryzae | methanol + gallic acid | - |
? | |
additional information | tannase activity is assayed by monitoring the production of gallic acid, released from methyl gallate, through reaction with rhodanine. The enzyme has no activity with the methyl esters of ferulic, p-coumaric, caffeic, and sinapic acids, or with the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid. Substrate specificity, overview | Aspergillus oryzae | ? | - |
? | |
additional information | tannase activity is assayed by monitoring the production of gallic acid, released from methyl gallate, through reaction with rhodanine. The enzyme has no activity with the methyl esters of ferulic, p-coumaric, caffeic, and sinapic acids, or with the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid. Substrate specificity, overview | Aspergillus oryzae RIB 40 | ? | - |
? | |
additional information | tannase activity is assayed by monitoring the production of gallic acid, released from methyl gallate, through reaction with rhodanine. The enzyme has no activity with the methyl esters of ferulic, p-coumaric, caffeic, and sinapic acids, or with the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid. Substrate specificity, overview | Aspergillus oryzae ATCC 42149 | ? | - |
? | |
propyl gallate + H2O | lower activity | Aspergillus oryzae | propanol + gallic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AoTanA | - |
Aspergillus oryzae |
TanA | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
assay at | Aspergillus oryzae |
General Information | Comment | Organism |
---|---|---|
additional information | the catalytic triad residues of AoTanA are predicted to be Ser195, Asp455, and His501, with the serine and histidine residues brought together by a disulfide bond of the neighboring cysteines, Cys194 and Cys502. Functional role of the Kex2 recognition sites and disulfide bond between the neighboring cysteines in enzyme AoTanA, overview | Aspergillus oryzae |