Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Benzene | activates by 15% at 20% | Staphylococcus lugdunensis | |
hexane | activates by 15% at 20% | Staphylococcus lugdunensis | |
additional information | non-polar organic solvents increase the tannase activity and polar solvents inhibit the tannase activity | Staphylococcus lugdunensis | |
SDS | activates at 1% by 20% | Staphylococcus lugdunensis | |
Toluene | activates by 50% at 20% | Staphylococcus lugdunensis |
Cloned (Comment) | Organism |
---|---|
gene tanA, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Staphylococcus lugdunensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | tannase immobilization provides protection of the enzyme over a wide range of pH and temperature. Immobilized tannase shows 80% activity up to seven cycles, more than 50% activity is observed even after 15 cycles | Staphylococcus lugdunensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | complete inhibition at 1% | Staphylococcus lugdunensis | |
DMSO | inhibits by 35% at 20% | Staphylococcus lugdunensis | |
EDTA | 15% inhibition at 1% | Staphylococcus lugdunensis | |
Fe2+ | inhibits at 5-10 mM | Staphylococcus lugdunensis | |
Fe3+ | inhibits at 5-10 mM | Staphylococcus lugdunensis | |
iso-amylalcohol | inhibits by 35% at 20% | Staphylococcus lugdunensis | |
K+ | inhibits at 5-10 mM | Staphylococcus lugdunensis | |
methanol | inhibits by 56% at 20% | Staphylococcus lugdunensis | |
Mg2+ | inhibits at 5-10 mM | Staphylococcus lugdunensis | |
Mn2+ | inhibits at 5-10 mM | Staphylococcus lugdunensis | |
additional information | non-polar organic solvents increase the tannase activity and polar solvents inhibit the tannase activity | Staphylococcus lugdunensis | |
Na+ | activates by 10% at 1 mM, and inhibits at 5-10 mM | Staphylococcus lugdunensis | |
Zn2+ | inhibits at 5-10 mM | Staphylococcus lugdunensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | activates by 10% at 1 mM, and inhibits at 5-10 mM | Staphylococcus lugdunensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus lugdunensis | Q0KKP0 | - |
- |
Staphylococcus lugdunensis MTCC 3614 | Q0KKP0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity | Staphylococcus lugdunensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
716 | - |
recombinant His6-tagged enzyme, pH 7.0, 37°C | Staphylococcus lugdunensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl gallate + H2O | - |
Staphylococcus lugdunensis | methanol + gallic acid | - |
? | |
methyl gallate + H2O | - |
Staphylococcus lugdunensis MTCC 3614 | methanol + gallic acid | - |
? | |
additional information | enzyme activity is estimated by using methyl rhodanine method | Staphylococcus lugdunensis | ? | - |
? | |
additional information | enzyme activity is estimated by using methyl rhodanine method | Staphylococcus lugdunensis MTCC 3614 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, recombinant His6-tagged enzyme, SDS-PAGE | Staphylococcus lugdunensis |
Synonyms | Comment | Organism |
---|---|---|
tan A | - |
Staphylococcus lugdunensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant His6-tagged enzyme | Staphylococcus lugdunensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | over 50% of maximal activity within this range, profile overview | Staphylococcus lugdunensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant His6-tagged enzyme | Staphylococcus lugdunensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | over 50% of maximal activity within this range, profile overview | Staphylococcus lugdunensis |