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Literature summary for 3.1.1.20 extracted from

  • Chaitanyakumar, A.; Anbalagan, M.
    Expression, purification and immobilization of tannase from Staphylococcus lugdunensis MTCC 3614 (2016), AMB Express, 6, 89 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Benzene activates by 15% at 20% Staphylococcus lugdunensis
hexane activates by 15% at 20% Staphylococcus lugdunensis
additional information non-polar organic solvents increase the tannase activity and polar solvents inhibit the tannase activity Staphylococcus lugdunensis
SDS activates at 1% by 20% Staphylococcus lugdunensis
Toluene activates by 50% at 20% Staphylococcus lugdunensis

Cloned(Commentary)

Cloned (Comment) Organism
gene tanA, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Staphylococcus lugdunensis

Protein Variants

Protein Variants Comment Organism
additional information tannase immobilization provides protection of the enzyme over a wide range of pH and temperature. Immobilized tannase shows 80% activity up to seven cycles, more than 50% activity is observed even after 15 cycles Staphylococcus lugdunensis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol complete inhibition at 1% Staphylococcus lugdunensis
DMSO inhibits by 35% at 20% Staphylococcus lugdunensis
EDTA 15% inhibition at 1% Staphylococcus lugdunensis
Fe2+ inhibits at 5-10 mM Staphylococcus lugdunensis
Fe3+ inhibits at 5-10 mM Staphylococcus lugdunensis
iso-amylalcohol inhibits by 35% at 20% Staphylococcus lugdunensis
K+ inhibits at 5-10 mM Staphylococcus lugdunensis
methanol inhibits by 56% at 20% Staphylococcus lugdunensis
Mg2+ inhibits at 5-10 mM Staphylococcus lugdunensis
Mn2+ inhibits at 5-10 mM Staphylococcus lugdunensis
additional information non-polar organic solvents increase the tannase activity and polar solvents inhibit the tannase activity Staphylococcus lugdunensis
Na+ activates by 10% at 1 mM, and inhibits at 5-10 mM Staphylococcus lugdunensis
Zn2+ inhibits at 5-10 mM Staphylococcus lugdunensis

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ activates by 10% at 1 mM, and inhibits at 5-10 mM Staphylococcus lugdunensis

Organism

Organism UniProt Comment Textmining
Staphylococcus lugdunensis Q0KKP0
-
-
Staphylococcus lugdunensis MTCC 3614 Q0KKP0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity Staphylococcus lugdunensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
716
-
recombinant His6-tagged enzyme, pH 7.0, 37°C Staphylococcus lugdunensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl gallate + H2O
-
Staphylococcus lugdunensis methanol + gallic acid
-
?
methyl gallate + H2O
-
Staphylococcus lugdunensis MTCC 3614 methanol + gallic acid
-
?
additional information enzyme activity is estimated by using methyl rhodanine method Staphylococcus lugdunensis ?
-
?
additional information enzyme activity is estimated by using methyl rhodanine method Staphylococcus lugdunensis MTCC 3614 ?
-
?

Subunits

Subunits Comment Organism
? x * 66000, recombinant His6-tagged enzyme, SDS-PAGE Staphylococcus lugdunensis

Synonyms

Synonyms Comment Organism
tan A
-
Staphylococcus lugdunensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
recombinant His6-tagged enzyme Staphylococcus lugdunensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 over 50% of maximal activity within this range, profile overview Staphylococcus lugdunensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
recombinant His6-tagged enzyme Staphylococcus lugdunensis

pH Range

pH Minimum pH Maximum Comment Organism
6 9 over 50% of maximal activity within this range, profile overview Staphylococcus lugdunensis